Can anyone help with detecting dopamine in SH-SY5Y cell lysate?

More Parijat Kabiraj's questions See All

How can we gate the Chemically measured well log data for the research?

Can any help me in this regards? I have some idea in my mind to calculate (Total organic Content) TOC from well logs using new technique. Please Help.

08 September 2023 7,031 0 View

How to differentiate Cellulose Iα and Iβ using XRD?

I am extracting cellulose from different land and aquatic biomass. I want to know if it is possible to differentiate cellulose Iα and Iβ using XRD.

30 July 2020 9,033 1 View

Mr. Bayes Tree shows very low posterior probability after 200 million run?

Hello Experts, I was running Mrbayes with 112 Diptera species, 4 out group of 13 concatenated mitochondrial genes comprising 11835 bp characters for 200 million generations. After that checked...

23 February 2020 5,090 2 View

Which version of DMEM, High Glucose (HG) or Low Glucose (LG), is recommended for culturing NIH 3T3 cells?

Hello everyone! I came across a lot of articles suggesting that HG-DMEM (containing 25mM D-Glucose) induces ROS in cells, and the authors have used cells grown in LG-DMEM (5mM D-Glucose) as...

01 October 2018 1,885 5 View

Robust cAMP assay kit for Gi coupled GPCRs ?

Dear all, Can anyone suggest me a robust cAMP assay kit for Gi coupled GPCRs? I would like to use this kit for cell lines stably expressing GPCRs that are coupled to Gi (inhibitory G proteins)...

16 October 2017 2,695 2 View

How to extract start codon and stop codon from a fasta of multiple protein coding genes ?

I want to know that is there is any suitable tool for extracting start codon and stop codon from the fasta file of multiple protein-coding genes (PCGs). I have downloaded multifasta PCGs...

02 April 2017 1,596 5 View

I would like to get an insight into my PCR problem?

Have two sets of primers to amplify two separate genes from the same genomic DNA template. I have been using fresh reagents. while one primer set is showing non-specific amplification (including...

15 January 2017 7,965 9 View

How do I predict Endogenous viral element (ERV and NRV) from desired host genome?

Is there any standard pipeline for such analysis?

19 April 2016 3,846 2 View

Can anyone advise me on the reaction conditions of methacrylol chloride with alcohol?

I want to prepare acrylic monomers from alcohols by reacting with methacrylol chloride. Can anyone provide me the reaction conditions or suggest me any literature where I can find that? Thanks in...

02 February 2016 4,784 3 View

How do I normalize my MTT assay for reduced cell number in treated sample if my drug inhibits cell division?

Hello All, I am doing a MTT assay where because of drug treatment , the no. of cells in treatment is reducing to half of control. Now, after MTT assay how do i know the reduction in absorbance is...

13 October 2015 802 11 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

In the situation below, can I apply braun and Clark thematic analysis?

I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).

04 August 2024 8,798 7 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

How are aPTT and PT values reduced after hemodialysis compared to before dialysis value in patients with chronic kidney disease?

I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...

30 July 2024 3,467 0 View

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program?

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...

28 July 2024 9,165 2 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

How can we separate diastereomers of larger organic moiety?

For example, oxazine type moieties which forms diastereomers, how the separation can be done? Chiral HPLC is the only possibility?

10 July 2024 1,519 3 View