Hello everyone! I came across a lot of articles suggesting that HG-DMEM (containing 25mM D-Glucose) induces ROS in cells, and the authors have used cells grown in LG-DMEM (5mM D-Glucose) as control. So far in my experience of culturing NIH 3T3 cells, I have used HG-DMEM without any problems. But now that I want to study ROS induction in NIH 3T3 cells, it would be great if anyone shared their views on the ideal glucose concentration in culture media. I have tried measuring ROS levels with DCFDA, induced by H2O2, and I required milimolar concentrations of H2O2 to observe visible change with respect to control cells. I am concerned if this is due to HG-DMEM. (I currently don't have LG-DMEM to use as control!)
these cells are robust and can grow easily in DMEM containing 5 mM glucose, If you use 25 mM glucose, cells will secrete relatively high amounts of lactic acid which may impair proliferation and metabolism relatively rapidly..
Hello Parijat,
According to recommended media by ATCC, you should use HG-DMEM as optimized growth media.
Best,
We noticed that the cells may enter into a stressed state if glucose is low.
Parijat low glucose media triggers ROS production in most cells. I do not know specifically for NIH3T3. However, you can go through the following paper for reference
https://www.sciencedirect.com/science/article/pii/S0891584911010665
I hope this helps.
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