Hello Experts,
I was running Mrbayes with 112 Diptera species, 4 out group of 13 concatenated mitochondrial genes comprising 11835 bp characters for 200 million generations. After that checked with tracer and ESS value is more than 200. But posterior probability support display very low, I have pasted the whole tree. What could be the reason for that..!!
((((O_oryzae:1.408894e-01,R_pomum:1.699315e-01):6.951337e-02,A_mellifer:3.461659e-01):4.381688e-02,(A_microrhi:4.444810e-01,E_biolleyi:2.896343e-01):8.282212e-02):0.02129044,(B_mori:1.686118e-01,((C_tepperi:1.325847e-01,P_vanderpl:1.151160e-01):8.117269e-02,((A_flava:1.493137e-01,C_arakawae:2.173195e-01):2.904698e-02,(((P_chinensi:8.621045e-02,P_papatasi:9.014292e-02):3.104472e-02,N_umbratil:8.703177e-02):9.925377e-02,((((O_vigilax:4.012775e-02,(H_janthino:4.727491e-02,A_notoscri:5.364228e-02):1.241852e-02):2.487020e-02,(C_pipiens:3.738753e-02,C_tritaeni:4.058987e-02):3.741806e-02):3.151311e-02,(((((A_culicifa:5.607482e-02,A_gambie:5.636795e-02):1.327441e-02,A_quadrima:7.003846e-02):7.408294e-03,(A_hinesoru:5.723663e-02,A_cracens:5.915450e-02):1.364204e-02):1.240354e-02,(A_deaneoru:1.608639e-02,((A_albitars:4.327825e-03,A_oryzalim:3.820422e-03):1.360588e-02,A_janconna:1.956103e-02):5.523806e-03):5.408099e-02):1.302482e-02,((A_cruzii:2.182612e-02,A_laneanus:2.291756e-02):1.021653e-02,(A_homuncul:3.567754e-02,A_bellator:3.718719e-02):6.935985e-03):4.970546e-02):3.225685e-02):5.772436e-02,((((((S_variegat:9.095285e-02,S_aureohir:7.694133e-02):7.986833e-02,(P_steineni:1.578956e-01,D_aestival:1.360293e-01):2.568001e-02):2.117294e-02,Ptychopter:1.222366e-01):1.768764e-02,((P_trichopt:1.562991e-01,T_bimacula:1.113396e-01):3.285489e-02,(S_hybrida:1.017936e-01,T_cockerel:1.617636e-01):3.302359e-02):1.515579e-02):1.458847e-02,((C_spenceri:1.682239e-01,P_fitchii:1.134891e-01):2.890889e-02,S_fenestra:1.358556e-01):1.899491e-02):1.810656e-02,(((C_duplonot:5.751269e-02,A_miser:5.293013e-02):5.825733e-02,T_punctata:1.703206e-01):2.304740e-02,((F_taylori:1.863463e-01,S_grandico:1.013370e-01):2.743123e-02,(((((Blepharipa:9.263945e-02,E_flavipal:6.600538e-02):2.907222e-02,(R_goerling:1.000130e-01,H_lineatum:1.275714e-01):1.959182e-02):1.310425e-02,((((((((A_grahami:1.332732e-03,C_chinghai:2.184006e-02):3.077199e-02,C_vomitori:2.527734e-02):2.220139e-02,((H_ligurrie:4.292224e-02,L_coerulei:5.016599e-02):7.412053e-03,(L_porphyri:3.857032e-02,(L_illustri:7.821766e-03,L_caesar:7.271867e-03):1.527432e-02):1.614499e-02):1.023368e-02):1.196501e-02,((((((((C_pinguis:5.741078e-03,C_phaonis:9.611718e-03):1.010053e-02,(C_saffrane:1.787382e-03,C_megaceph:1.691729e-03):1.173043e-02):1.008060e-02,C_bezziana:2.884081e-02):1.970567e-02,C_putoria:3.003025e-02):8.210260e-03,C_nigripes:3.814942e-02):7.819057e-03,P_terraeno:4.840502e-02):9.815889e-03,(C_albiceps:1.693157e-02,C_rufifaci:1.744351e-02):2.757707e-02):1.159690e-02,C_hominivo:6.725860e-02):8.902556e-03):1.053225e-02,(R_pernix:4.354929e-02,((S_melanura:4.269643e-02,((S_africa:4.431724e-02,S_crassipa:4.028260e-02):1.085658e-02,((S_portschi:4.507423e-02,S_similis:3.117872e-02):6.569835e-03,(S_peregrin:5.088085e-02,S_albiceps:3.457433e-02):7.528131e-03):6.463800e-03):6.396419e-03):1.100554e-02,S_impatien:6.104595e-02):2.102877e-02):3.870008e-02):9.177258e-03,(S_stercora:4.268777e-02,D_antiqua:6.176151e-02):1.618036e-02):1.378860e-02,((M_stabulan:6.033913e-02,M_levida:6.496915e-02):4.936369e-02,H_irritans:5.701933e-02):1.542006e-02):1.137556e-02,(D_hominis:8.766363e-02,(G_pecorum:8.335484e-02,G_intestin:8.955520e-02):1.172282e-01):3.542531e-02):8.366930e-03):2.980716e-02,(((((D_incompta:7.016329e-02,D_littoral:6.826161e-02):2.805877e-02,D_formosan:4.177684e-02):2.086597e-02,D_pseudoob:7.310143e-02):1.106976e-02,(D_santomea:4.223774e-02,(D_melanoga:2.489059e-02,D_mauritia:2.434300e-02):1.746964e-02):1.600782e-02):3.637594e-02,E_sorbilla:7.254971e-02):3.169820e-02):1.955885e-02,((((L_huidobre:4.956470e-02,L_bryoniae:5.029102e-02):3.854880e-02,L_sativae:7.249095e-02):5.349794e-02,M_sojae:1.174303e-01):4.654720e-02,(N_mamaevi:1.407510e-01,(P_utilis:1.308985e-01,((((B_zonata:3.248505e-02,B_correcta:3.389673e-02):1.812366e-02,((((B_philippi:4.386213e-03,B_papayae:4.581949e-03):9.802061e-04,B_carambol:7.213513e-03):3.366193e-03,B_invadens:4.859007e-03):3.800487e-02,(B_umbrosa:9.656220e-02,B_arecae:5.268648e-02):1.806720e-02):8.690348e-03):1.190069e-02,B_melastom:5.102569e-02):3.743031e-02,(((B_scutella:4.033945e-02,B_diaphora:3.003100e-02):3.385232e-02,B_tau:5.975168e-02):2.462184e-02,(D_longicor:9.696546e-02,B_minax:1.483224e-01)OROOT:3.622887e-02):2.398196e-02):5.804132e-02):2.466830e-02):1.971457e-02):1.443621e-02):1.635323e-02):2.314916e-02):2.332569e-02):2.036662e-02):2.634711e-02):1.928994e-02):2.319814e-02):4.316914e-02):0.02129044);
Thanks,
Debajyoti
Hello,
I think the tree you provided includes only the branch length, but not including the posterior probability support value.
Please refer to the sumt command in MrBayes v3.2 and open the resulting .con.tre file with the FigTree software or a similar software.
Good luck!
Clearly your data is insufficient to resolve the tree. It is not phylogenetically informative.
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