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Questions related from Gustavo Orlando Bonilla-Rodriguez
Hi there, sometimes we have trouble dissolving 1 % (w/v) birchwood xylan for use as a substrate for xylanases. Any suggestions?
02 February 2016 8,075 5 View
Hi, we need to remove tween from a protein solution that will be analyzed by MS. What would be the available options starting by those cheap, effective and simple? Thanks!
09 September 2014 9,248 6 View
I'm looking for available options for chromogenic substrates for pectinases; I would need, for example, 4-Nitrophenyl-Alpha-D-Galacturonide, but this substrate seems hard to find . Sigma-Aldrich...
12 December 2013 3,100 4 View
Salting-out is related to the hydrophobic patches close to the protein surface and the removal of the hydration layers around proteins. For glycoproteins that are "hard to precipitate", could the...
11 November 2013 8,272 2 View
We'd like to try a chemical approach to check whether some enzymes that apparently are isoforms, could be in fact just a single protein with different degrees of glycosylation. For that we need a...
10 October 2013 2,912 0 View