Hi,

could you please provide the "sizes" of MW Marker bands?

Hi,

From the bottom

250

500

750

1000

1500

...

Hi

Do the following and attach picture. though at 500bp your digested product is not visible.

lane 1: Marker. Lane 2 plasmid without digestion, lane 3 plasmid digested with xba only, lane 4 plasmid with bamhi, and lane 6 with double digestion, lane 7 if u have a positive clone having same restriction sites (ask any one in your lab).

Use restriction buffer as per recomendation of the company you are using.

i have assumed you have confirmed your clone by pcr..

if possible confirm more colonies.

Hope so this will help you.

Perhaps a silly comment but you have checked that Xba and BamHI don't cut your insert?