Victoria Gillan

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Questions related from Victoria Gillan

Matrigel domes disappearing? Not polymerizing at 37?

Hi there, I am having problems with 50ul Matrigel domes completely dissolving in 24 well plates. I am trying to generate organoid cultures, but when I remove the culture media after 2 days the...

15 September 2021 8,502 5 View

MiRNA Target prediction sites - recommendations?

Hi there, I have resurrected an old project which involved looking at mRNA targets of a miRNA in a parasitic nematode. As it isn't a model organism, TargetScan is no use. We used to use PITA and...

02 June 2020 3,401 0 View

PITA miRNA binding prediction sites?

Until recently I relied on the Online microRNA prediction tool which uses the PITA algorithm https://genie.weizmann.ac.il/pubs/mir07/mir07_prediction.html . The Weizmann lab are no longer...

18 January 2018 4,532 3 View