Can anyone suggest me AI and WSN integrated Simulators and Emulators which are more suitable for real time applications?

For building the prototype of any of the real-time applications, the algorithms mostly are AI based AI-based. Hence, it would be better if the integrated tool is available to develop the...

07 June 2020 8,580 6 View

How to avoid dissolved gasses in Ni base alloy melting practice?

Ni with 3%Si tapped at 1300 degree Celsius shows bubbles structure on the Riser top.

23 November 2018 348 3 View

Help me to identify German Project leader?

i am planning to apply for a Indo-German Collaborative Research Activities in Materials Science and Engineering in DST.the research field is organic solar cells.

18 September 2018 8,919 2 View

How do I store/handle the polymeric electrospun scaffolds?

I have been producing polycaprolactone fibers by means of electrospinning on an aluminium foil onto a metallic collector plate. After the polymer fibers have deposited on the foil, I remove it...

24 April 2017 7,903 3 View

What is the best method used to align the diffractometer plane/axis with respect to the incident beam direction?

Most of the XRD community is presently using 0D point counter or 1D linear PSD (position sensitive detector) in powder diffraction as well as rocking curve analyses. Any mention of the extinct...

02 December 2016 5,307 3 View

How do I calibrate the diffractometer 2Theta angles with this powder diffractogram of corundum standard from a Bruker D8 XRD instrument?

04 November 2016 4,212 4 View

How do I compute b, Burgers Vector correctly using the Hirsch approach to computing dislocation density employing XRD rocking curve profile analysis?

19 September 2016 2,394 8 View

SiC 6H - What periodicity would create a Debye-Scherrer arc at about 2 degrees 2-Theta using Cu K Alpha 1 only in HRXRD observation -1 to +3 degrees?

While collecting XRR data (-1 to +3 degrees 2Theta) for a SiC 6H 75mm diameter wafer, a reflection was observed at about 2 degree 2-Theta on the 2D detector as well as the XRR Omega-2Theta scan....

12 May 2016 8,437 2 View

Are there case studies on larger adoption of agriculture technologies after adaptive trails at farmers field?

An adaptive trail result that can make larger impact for more communities to adopt

03 March 2016 3,757 5 View

Is there a unit of mass flow rate/volume flow rate by the name "Stoc"? How it may be converted into SI units?

Must be some old unit of measurement.

07 December 2015 3,795 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View

How to remove unreacted florescent dye from labeled protein?

Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...

02 February 2021 644 2 View