Can anyone help with a basic mechanism regarding monitoring intracellular calcium with Fluo-3/4 AM using Flow cytometry?

More Yue Liu's questions See All

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View

How to evaluate teachers' professional vision?

science teachers' professional vision:framewor,evaluation,review of related research,researchers related,research method,why professional vision

20 July 2024 6,577 1 View

The role of binocular diplopia in the dental treatment of visual field defects?

In dentistry, we sometimes need ‘binocular diplopia’ when dealing with complex dental problems, which means using different angles and tools to ensure the treatment is effective. In your opinion,...

09 July 2024 3,898 0 View

How to disperse silverparticles in water?

I synthesize silver nanoparticles ，and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...

09 July 2024 7,335 0 View

Interactions between oral microbiota and oral sensory systems: impact on oral comfort and pain perception?

This research question explores the complex interactions between oral microbes and the oral sensory system. The diversity and function of oral microbial communities play an important role in oral...

09 July 2024 3,601 0 View

Zero pivot in Abaqus concrete 3D printing simulation using model change?

Recently I've been working on a simulation of concrete 3D printing, and has successfully simulated a layer-by-layer 3D printing process using model change. However, when I proceeded to try...

06 July 2024 2,130 0 View

TCAD how should i define this Doping Dependece?

The material is SiGe

03 July 2024 1,374 0 View

Why did the simulated load-displacement curve of beam keep increasing and didn't decrease (ABAQUS)?

Dear colleagues, I'm currently modelling a simple 4-point bending test of a concrete beam in Abaqus. However, the load-displacement curve was ascending (without final point), which didn't match...

29 June 2024 6,192 2 View

Sentaurus Tcad undefined model error whats problem?

I already used SiliconGermanium.par why it still error someone can explain this thank you!!

27 June 2024 9,269 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Edward J Roy

Molecular Probes has a "No Wash" version of Fluo-4 that works well.

Yue Liu

Thanks, Edward. Do you think there is a chance that the Fluo-4 bonds on the extracellular ca2+ and read by the flow?

Xiaowei Hou

If the fluo-4 is the AM version, it shouldn't read the extracellular Ca2+ due to lack of esterase.