Can anyone help me with direct article on sex determination in spiders using flow cytometry?

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Olivier Catrice

What are the mechanisms of sex determination in spiders ?

Are there sexual chromosomes like in insects, birds and mammals ?

If so there may be a difference in the Genome Size/%GC between male and female.

To measure %GC you will have to use Hoechst 33342 (%AT) or Mithramycin/ Chromomycin A3 (%GC).

Problems come with particular excitation of these fluorochromes : 365 nm for Hoechst and Mithramycin, 458 nm for Chromomycin.

Nicole D Riddle

A Google search found this:

Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species

What exactly are you looking for?