I am using the following protocol for making TBE 10x:
TBE 10X : 4 cc
acrylamide: 8 cc
APS: 600 microliter
TEMED: 40 microliter
voltage: 200w for 4 hours
I have applied the same formula for a year and there was no problem. but, recently I am observing bands and the marker scattered all over the gel. and there is no sharp interpretative band. I myself guess it might be due to voltage fluctuation.