I need to develop a portable low cost sensor using a nitrocellulose membrane (NM). I have done an assay for my analyte on a 96 well plate using monoclonal and polyclonol antibodies and a labeling agent. How do i translate this onto a NC membrane? What are the practical issues that I need to take care while dealing with NC membrane ? What are the step by step sample and NC membrane preparation methods, if any ? Are there any treatment procedures ? What is the simple assay being generally followed for NC membrane ?

Thanking you