19 Questions 41 Answers 0 Followers
Questions related from Gangula Abilash
Hi, I am trying to develop a competitive lateral flow assay to detect a protein. I coat the protein on the test line and use gold nanoparticle-antibody as the conjugate that binds to the test...
02 February 2019 4,072 5 View
Hi, I am trying to synthesize ultra small 2nm gold nanoparticles by NaBH4 reduction (Brust Method). What should be the characteristic UV-Vis spectrum of 2nm size gold nanoparticles. Should we or...
09 September 2018 3,181 10 View
Hi I was doing an organic syntheis. I was supposed to obtain the product as a white powder. Instead, I was getting an yellowish and bit oily and sticky product. I am giving below the procedure I...
08 August 2018 9,690 4 View
Hello Everyone I was trying to measure a protein in human urine samples using ELISA. When I was doing spike-recovery experiment, the recovery is less than 50 %, indicating that urine matrix is...
03 March 2018 1,444 4 View
We are trying to detect a Virus using a sandwich lateral flow assay. We are targeting the epitopes on the envelop protein of the virus. Is there anyway I can inactivate the disinfect the virus,...
03 March 2018 4,255 3 View
I am trying to develop a gold nanoparticle based lateral flow sensor to detect a whole intact Virus. I have tried with 3 different antibodies of the virus. But, I was not successful. Reading the...
02 February 2018 382 9 View
Suppose a virus has 3 different strains. Will the antibody specific to the virus show affinity for all the 3 strains ?
10 October 2017 1,977 3 View
Hello Everyone, I am trying to create -SH groups in cetuximab antibody by reacting it with Traut's reagent. I followed the procedure given by the protocols of Thermo...
01 January 2017 2,167 16 View
I would like to know whether ammonium per sulfate (APS) is a good radical initiator for synthesizing PMMA nanoparticles by emulsion radical polymerisation. Or, does it have any drawbacks...
10 October 2016 5,780 9 View
What is the mechanism involved in silver enhancing the color intensity of gold nano particles in a lateral flow assay ? What is the reagent that we use for that ? Is it silver salt solution with a...
08 August 2016 305 1 View
Can anyone who worked with quantum dots suggest what be the ideal quantum dot to use for paper based lateral flow assay. I should be able to conjugate antibody to it and we are looking for a...
08 August 2016 3,712 3 View
I need to develop a portable low cost sensor using a nitrocellulose membrane (NM). I have done an assay for my analyte on a 96 well plate using monoclonal and polyclonol antibodies and a labeling...
06 June 2016 979 1 View
What is the anatomy of nitrocellulose (NC) membrane ? Is it a kind of 3-D network with pores in between ? When we are blocking a nitro cellulose membrane, What are we actually doing ? Are we...
06 June 2016 6,688 2 View
We know that nucleobases like guanine and cytosine complementarily bind to each other via hydrogen bonds in a DNA helix. Will this H-bonding take place between these molecules when taken...
05 May 2016 9,620 4 View
Can anyone explain the physics behind why graphene fundamentally does not have any band gap? Also can anyone send a good reading material regarding the same?
03 March 2014 329 2 View
In a UV-vis apectrophotometer, what does it mean if the absorption spectrum is coming in the negative region of the y-axis (absorbance) even after taking a baseline with reference?
12 December 2013 1,970 22 View
I want to dissolve a compound by sonicating it in an appropriate solvent as I was not able to dissolve it completely otherwise. Does sonication denature the compound?
11 November 2013 4,301 8 View
I am trying to do a gold nanoparticle (AuNP) based lateral flow assay for detecting an analyte. It is a competitive assay, where in the conjugate pad has the AuNP-Antibody conjugate, the...
01 January 1970 304 13 View
I have to develop a paper strip for detection of a small molecule, a nucleoside. I need some suggestions on the arrangement of different pads as I am doing the paper based sensor for the first...
01 January 1970 5,560 7 View
