Gangula Abilash

19 Questions 41 Answers 0 Followers

Questions related from Gangula Abilash

Color at the test line of a lateral flow imuunoassay strip ?

Hi, I am trying to develop a competitive lateral flow assay to detect a protein. I coat the protein on the test line and use gold nanoparticle-antibody as the conjugate that binds to the test...

02 February 2019 4,064 5 View

What is the characteristic UV-Vis Spectrum of Ultra small Gold Nanoparticles ?

Hi, I am trying to synthesize ultra small 2nm gold nanoparticles by NaBH4 reduction (Brust Method). What should be the characteristic UV-Vis spectrum of 2nm size gold nanoparticles. Should we or...

09 September 2018 3,175 10 View

What causes a product to change color and turn sticky during an organic synthesis ?

Hi I was doing an organic syntheis. I was supposed to obtain the product as a white powder. Instead, I was getting an yellowish and bit oily and sticky product. I am giving below the procedure I...

08 August 2018 9,684 4 View

How to avoid urine matrix interference while measuring proteins using an ELISA?

Hello Everyone I was trying to measure a protein in human urine samples using ELISA. When I was doing spike-recovery experiment, the recovery is less than 50 %, indicating that urine matrix is...

03 March 2018 1,438 4 View

What are the ways to inactivate a Virus without affecting its ability to bind to its antibody ?

We are trying to detect a Virus using a sandwich lateral flow assay. We are targeting the epitopes on the envelop protein of the virus. Is there anyway I can inactivate the disinfect the virus,...

03 March 2018 4,249 3 View

How much more difficult it is to detect a whole virus when compared to its Non Structural (NS) Protein ?

I am trying to develop a gold nanoparticle based lateral flow sensor to detect a whole intact Virus. I have tried with 3 different antibodies of the virus. But, I was not successful. Reading the...

02 February 2018 376 9 View

Are antibodies of a Virus specific strain ?

Suppose a virus has 3 different strains. Will the antibody specific to the virus show affinity for all the 3 strains ?

10 October 2017 1,970 3 View

How the reaction of an Antibody with Trauts Reagent to create -SH groups can be made succesfull ?

Hello Everyone, I am trying to create -SH groups in cetuximab antibody by reacting it with Traut's reagent. I followed the procedure given by the protocols of Thermo...

01 January 2017 2,161 16 View

Is there any ideal initiator for synthesis of PMMA Nanoparticles through emulsion radical polymerisation ?

I would like to know whether ammonium per sulfate (APS) is a good radical initiator for synthesizing PMMA nanoparticles by emulsion radical polymerisation. Or, does it have any drawbacks...

10 October 2016 5,772 9 View

How does silver staining increase the intensity of colorimetric signal of gold nanoparticles in a lateral flow assay?

What is the mechanism involved in silver enhancing the color intensity of gold nano particles in a lateral flow assay ? What is the reagent that we use for that ? Is it silver salt solution with a...

08 August 2016 299 1 View

What Quantum dots would be ideal for highly sensitive paper based lateral flow assay?

Can anyone who worked with quantum dots suggest what be the ideal quantum dot to use for paper based lateral flow assay. I should be able to conjugate antibody to it and we are looking for a...

08 August 2016 3,704 3 View

Can anyone help me with designing a portable sensor using a nitrocellulose membrane ?

I need to develop a portable low cost sensor using a nitrocellulose membrane (NM). I have done an assay for my analyte on a 96 well plate using monoclonal and polyclonol antibodies and a labeling...

06 June 2016 972 1 View

What is the actual mechanism involved in blocking a nitrocelluolse membrane ?

What is the anatomy of nitrocellulose (NC) membrane ? Is it a kind of 3-D network with pores in between ? When we are blocking a nitro cellulose membrane, What are we actually doing ? Are we...

06 June 2016 6,682 2 View

Does hydrogen bonding between two complementary molecules happen in water ?

We know that nucleobases like guanine and cytosine complementarily bind to each other via hydrogen bonds in a DNA helix. Will this H-bonding take place between these molecules when taken...

05 May 2016 9,614 4 View

Why doesn't graphene have any band gap?

Can anyone explain the physics behind why graphene fundamentally does not have any band gap? Also can anyone send a good reading material regarding the same?

03 March 2014 323 2 View

Any advice for absorption spectrum coming in the negative region of the y-axis in a UV-vis spectrophotometer?

In a UV-vis apectrophotometer, what does it mean if the absorption spectrum is coming in the negative region of the y-axis (absorbance) even after taking a baseline with reference?

12 December 2013 1,962 22 View

Does sonication denature a compound?

I want to dissolve a compound by sonicating it in an appropriate solvent as I was not able to dissolve it completely otherwise. Does sonication denature the compound?

11 November 2013 4,294 8 View

Is Lateral Flow Assay specific to clonality of the anitbody ?

I am trying to do a gold nanoparticle (AuNP) based lateral flow assay for detecting an analyte. It is a competitive assay, where in the conjugate pad has the AuNP-Antibody conjugate, the...

01 January 1970 298 13 View

Can anyone help me with design of paper strip for lateral flow immunoassay ?

I have to develop a paper strip for detection of a small molecule, a nucleoside. I need some suggestions on the arrangement of different pads as I am doing the paper based sensor for the first...

01 January 1970 5,554 7 View