How to detect unbinding of a protein from a gold nanoparticle through UV-Vis?

From literature, it could be observed that while binding of gold nanoparticle and protein occurs the UV-VIS peak of gold shifts by a small amount suggesting binding. Although further...

15 October 2018 8,667 3 View

Does binding of a protein to a gold nanoparticle change the color of gold nanoparticles?

Binding of a protein to a gold nanoparticle(5-7 nm) increases the size of the complex as validated by a red shift in the uv-vis spectra. Does the overall size increase of AuNP-Protein complex...

30 July 2018 7,770 3 View

I want to know, How can i calculate the mixture of solvent in gaussian 09?. How will i specified the mixture of solvents in gaussiaan inputs?

I want to know, How can i calculate the mixture of solvent in gaussian 09?. How will i specified the mixture of solvents in gaussiaan inputs? Please anyone can help.....Thanks in advance

26 January 2016 4,513 2 View

What is the example of IF ring which is not von Neumann regular?

22 April 2015 4,897 1 View

How can I calculate an equivalent dose (in vitro to in vivo)?

15 August 2014 8,174 4 View

Can anyone help me to suggest using 4 colors for Immunofluorescent including nuclear staining?

Currently I am working on one protein with 2 different variants, it is basically nuclear localization, but there is no clue about the 2 variants. If I checked individually means either one may be...

14 August 2014 9,529 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

Why is my protein not binding to my reversed-phase column?

Hello everyone! Short facts: I am trying to perform reversed-phase chromatography via a desalting column (Waters: Sep-Pak tC18 3cc) of an already purified hydrophobic protein to exchange the...

01 February 2021 5,471 15 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Why does the DNA's elution decrease after centrifuging with spin column?

02 January 2021 987 4 View