Beverley Marie Rabbitts

6 Questions 14 Answers 0 Followers

Questions related from Beverley Marie Rabbitts

Microbial microplate transparent seal?

Hi, I'm looking for a product recommendation. I want to seal microplates containing bacteria for an overnight growth curve at 30-37 degrees C. The job of the seal is to keep moisture from escaping...

06 November 2022 297 3 View

How long can you store fluorescence microscopy samples in PBS?

Hi, I am taking over a project that has many interesting microscopy samples stored in PBS + sodium azide for various number of months or years. I want to know how old can immunofluorescence...

18 June 2021 1,102 2 View

Best way to get rid of SDS before LC-MS-MS without losing hydrophobic peptides?

There are many methods to get rid of SDS from a protein sample, after trypsin digestion, before LC-MS-MS. These include precipitation (TCA and/or cold acetone), spin columns designed for detergent...

18 September 2015 3,622 9 View

Where is an online database of escape mutations evolving after chemical mutagenesis?

Hi, I am sure somebody has done chemical mutagenesis (e.g. ENU), to introduce small but harmful mutation(s), followed by a second round of mutagenesis and selection for escape mutation(s)...

14 July 2015 6,406 1 View

Is there any genetically encoded site-specific proximity labeling?

I would like to find out if there exists a chemical probe that can be targeted to a specific site on a protein, that has been genetically labeled without any unnatural amino acid incorporation,...

03 April 2015 121 5 View

If you have two secondary antibodies raised in the same type of animal, can you stain simultaneously with them?

For example, you want to view protein A and protein B by immunofluorescence microscopy in a mouse cell. So you get rabbit anti-A and mouse anti-B. They you get green-labeled goat anti-rabbit and...

12 December 2014 7,092 8 View