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Questions related from Beverley Marie Rabbitts
Hi, I'm looking for a product recommendation. I want to seal microplates containing bacteria for an overnight growth curve at 30-37 degrees C. The job of the seal is to keep moisture from escaping...
06 November 2022 306 3 View
Hi, I am taking over a project that has many interesting microscopy samples stored in PBS + sodium azide for various number of months or years. I want to know how old can immunofluorescence...
18 June 2021 1,113 2 View
There are many methods to get rid of SDS from a protein sample, after trypsin digestion, before LC-MS-MS. These include precipitation (TCA and/or cold acetone), spin columns designed for detergent...
18 September 2015 3,628 9 View
Hi, I am sure somebody has done chemical mutagenesis (e.g. ENU), to introduce small but harmful mutation(s), followed by a second round of mutagenesis and selection for escape mutation(s)...
14 July 2015 6,418 1 View
I would like to find out if there exists a chemical probe that can be targeted to a specific site on a protein, that has been genetically labeled without any unnatural amino acid incorporation,...
03 April 2015 126 5 View
For example, you want to view protein A and protein B by immunofluorescence microscopy in a mouse cell. So you get rabbit anti-A and mouse anti-B. They you get green-labeled goat anti-rabbit and...
12 December 2014 7,109 8 View
If GFP is fused to a mitochondrial targeting signal peptide, we know it gets expressed and imported into the mitochondrion, where its fluorescence can be visualized. But we also know that proteins...
04 December 2014 2,663 4 View
