I have used both viral supernatant as well as viral particles concentrated by ultracentrifugation to infect EA.hy926 cells. But I have been unable to transduce them. The same viral particles have been able to efficiently transduce other cell lines. I have tried both 8ug/ml and 10ug/ml polybrene, but have not been able to transduce the cells. Can someone please help me out?

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