Amrutha Manikandan

2 Questions 2 Answers 0 Followers

Questions related from Amrutha Manikandan

What is a good starting concentration for phosphoproteomics?

I am performing whole cell phosphoproteomics. Following cell lysis, I have trypsinized and desalted 10mg of cell lysate, which I am using for phosphoenrichment. The enrichment is antibody based,...

27 June 2022 9,984 4 View

Can anyone help me optimize Lentiviral transduction in EA.hy926 cells?

I have used both viral supernatant as well as viral particles concentrated by ultracentrifugation to infect EA.hy926 cells. But I have been unable to transduce them. The same viral particles have...

14 September 2021 662 1 View