Does de-ionized water change color of a substrate coated with some chemical?

Out of curiosity, I would like to know whether any chemical coating on a substrate change its color when de-ionized water touches it. Precisely, if I pass some water through a microchannel in...

22 October 2016 4,945 3 View

Is Bi2Te3 a type of Topological Insulator an antiferromagnetic material or not?

17 October 2016 7,534 2 View

Why is there no velocity in output for harmonic analysis in ABAQUS?

I modeled a simple rectangular plate in ABAQUS to perform a harmonic analysis with 1N force at the centre fixed on the edges and used 'Steady state dynamics, Direct'. But I get '0' velocity...

09 August 2016 5,360 5 View

Do ANSYS APDL and workbench produce same modal results for a particular problem? If not what is the error range?

I am trying to perform a Modal analysis on a thin circular plate. But the results on both the platforms do not match. Am i doing something wrong?

23 May 2016 8,075 3 View

Any advice on the force experienced by a wall caused by a moving vortex?

20 December 2015 4,845 5 View

Can anyone help me in the synthesis of 2 amino 1,3,4- thiadiazole from thiosemicarbazone?

Cyclization of thiosemicarbazone to 2 amino 1,3,4- thiadiazole methods from aromatic aldehydes.

29 April 2014 3,242 5 View

How to perform cyclic voltammetry using the 3-electrode setup in 0 V to -0.8 V range in basic electrolyte?

I have to check CV characteristic of my pseudocapacitor electrode which was made using reduced graphene oxide and ZnO nano particles. I am using Ag/AgCl as reference electrode, Pt foil as a...

01 January 1970 5,749 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View