Does anyone has any experience in measuring thymosin beta 4 protein expression using western blot?

We are trying to measure thymosin beta 4 protein expression in HUVEC cell line and in mouse muscle and brown adipose tissue using western blot technique. However, we are not getting any band or...

07 December 2017 3,786 3 View

I'm looking for a cheap anionic dye, something maybe as expensive of fluorescein. The few I have found are quite expensive. Any advice?

Working on a project currently that requires the use of an anionic dye. Many of the ones I have found are quite expensive - on the order of $200 for 5g (sulfrhodamine etc). Do you guys have some...

20 June 2017 3,455 3 View

Does de-ionized water change color of a substrate coated with some chemical?

Out of curiosity, I would like to know whether any chemical coating on a substrate change its color when de-ionized water touches it. Precisely, if I pass some water through a microchannel in...

22 October 2016 4,945 3 View

Is Bi2Te3 a type of Topological Insulator an antiferromagnetic material or not?

17 October 2016 7,534 2 View

Why is there no velocity in output for harmonic analysis in ABAQUS?

I modeled a simple rectangular plate in ABAQUS to perform a harmonic analysis with 1N force at the centre fixed on the edges and used 'Steady state dynamics, Direct'. But I get '0' velocity...

09 August 2016 5,360 5 View

Do ANSYS APDL and workbench produce same modal results for a particular problem? If not what is the error range?

I am trying to perform a Modal analysis on a thin circular plate. But the results on both the platforms do not match. Am i doing something wrong?

23 May 2016 8,075 3 View

Any advice on the force experienced by a wall caused by a moving vortex?

20 December 2015 4,845 5 View

How can we convert a linear azide into an amine?

The easiest way to convert linear azide into amine.

26 November 2014 4,776 17 View

Can anyone help me in the synthesis of 2 amino 1,3,4- thiadiazole from thiosemicarbazone?

Cyclization of thiosemicarbazone to 2 amino 1,3,4- thiadiazole methods from aromatic aldehydes.

29 April 2014 3,242 5 View

What is the advantage of using 5 % milk in TBS-T as a blocking agent over 5 % BSA (Bovine Serum Albumin) in TBS-T

Which is the best blocking agent among BSA and Milk? Does it vary depending on the type of protein?

19 January 2014 1,098 35 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View