Photoionisation cross section of (singly) excited Helium?

Hello, I am searching the photoionisation cross section of (singly) excited Helium (1s2s as well as 1s3s). I only find either neutral Helium or doubly excited Helium. If someone could provide...

17 November 2020 7,096 3 View

If the daily (hourly) concentration of PM2.5 is not Gaussian. Is correct apply an arithmetic mean in epidemiological studies for risk assessment?

The distribution of daily PM2.5 concentration (hourly) is bimodal in the city of Coyhaique (South of Chile). Coyhaique is the city more polluted by particulate matter accord the WHO. The major...

11 October 2020 2,006 3 View

Can mycoplasma contamination in primary neuronal culture interfere with neuronal growth?

I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...

30 September 2020 2,517 1 View

Does anyone have experience with the Menstrual Attitude Questionnaire?

I am considering of using the original one from Brooke-Gunn and Ruble (1980), but maybe there is something better? I am trying to analyze the attitude of athletes towards menstruation and its...

27 August 2020 6,541 2 View

Residual stresses of heat-soaked hot bent glass - are they different from flat glass?

Usually, during the structural design of flat glass, one does not need to take into account the effects of changes from residual stresses. Question: How to understand and predict the residual...

15 March 2020 681 5 View

The compensation procedure- beads (for fluorochrome) and cells (dead/live staining)- how to do it?

Hi, I would like to ask about the compensation procedure. I use Fortessa/ Diva and automated compensation procedure. I have a set from BD for compensation setting. Referring to the manufacturer...

28 February 2020 8,765 2 View

Structural steel design - Effective length of a fillet weld according to EN 1993-1-8, 4.5.1 (1) unclear in definition?

In contrast to the older German steel code DIN 18800-1, the Eurocode for steel design defines an effective length of a fillet weld as follows (no sketch is provided in the Eurocode): "The...

12 December 2019 592 5 View

What standardised psychometric test to use for self-efficacy for female athletes?

Hello, I am looking for a valid and standardised test to measure self-efficacy of female athletes for my doctoral thesis. Any suggestions? Thank you

14 November 2019 849 3 View

What is the best recommended growth and freeze media for SH-SY5Y cells?

I received SH-SY5Y WT cells and started growing them in low glucose DMEM with FBS and S/P. After reading article about changed cell morphology and detachment of cells from growth surface due to...

06 October 2019 1,475 2 View

How is the deflection of aluminum frame structures influenced by the stiffness of corner cleats and the fitting tolerance?

How is the deflection of aluminum frame structures influenced by the stiffness of corner cleats and the fitting tolerance? Explanation: An ( orthogonal ) corner where two (extruded) aluminum...

05 June 2019 2,194 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View