Can anyone help me identify a cell culture contamination?

31 March 2023 1 9K Report

cell culture: HEK293F

medium: BalanCD, without antibiotics

Are you referring to those fibrous sheets? looks like some film that had dried onto the bottom of the well is peeling off as it has been hydrated.

Badges
Science method

More Dominika Franz's questions See All

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

How many search hits is enough for scoping review?

I am deciding on search terms for a scoping review. I am planning searching 3 multidisciplinary databases and currently getting about 5000 hits from my search terms in the first database. What do...

19 June 2024 7,417 0 View

RQ results discrepancy?

Hello, I'm relatively new to qPCR and have been utilizing a manufacturer's analysis software for my experiments, including one-way ANOVA for statistical analysis. However, I've encountered a...

14 May 2024 3,307 3 View

What test should be used for comparing multiple groups of categorical variables with counts per group <5?

Chi2 should be used for groups which count at least 5. What if one of the four groups compared is smaller? Additional question: any post-hoc test for comparison between groups? Thank you!

13 May 2024 1,425 2 View

Problem: cell aggregation with Cytofix/Cytoperm BD Biosciences?

The challenge encountered lies in cellular aggregation and clumping subsequent to incubation with Cytofix/Cytoperm BD Biosciences. Despite attempts to mitigate the issue through pipetting, the...

12 March 2024 3,926 0 View

Is there a technique, MRI sequence, for tracing spinal nerve root lesions in multiple sclerosis?

More recently, trigeminal nerve lesions related to multiple sclerosis found much attention. In contrast, scarcely a report has focused on how MS affects posterior or anterior spinal nerve roots....

09 September 2023 9,249 2 View

Can pressure monitoring devices be small enough to be riveted to central vein walls ?

No one knows how high and fast central venous pressures maximally rise - even in animals. The question attains special relevance for ill-collateralized vessels in the periphery of obstructed large...

10 May 2023 4,884 0 View

How can I prepare Rietveld Refinement for two compunds?

In my case: LiBH4 - LiBr in different molar ratio. I have the observed patterns (measured by XRD technique) for this mixture of compounds but I don't have .cif file or any reference to compare...

03 March 2023 2,640 4 View

How to eliminate dark background in DAB stains of mouse brain cuts?

Hi, I recently started doing a lot of IHC and I’ve been staining mouse coronal section (not in parafin) with 3D6 antibody to visualize plaques. My problem is that the background is too dark and...

02 December 2022 6,768 1 View

Which technique allows to show how high peripheral, or central venous pressures do peak?

The round-the-clock monitoring of arterial pressures has several serious shortcomings. Round-the-clock monitoring of the pressure rises in the periphery of ill-emptying central veins seems not...

16 September 2022 1,965 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View