Can mycoplasma contamination in primary neuronal culture interfere with neuronal growth?

I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...

30 September 2020 2,517 1 View

Does anyone have experience with the Menstrual Attitude Questionnaire?

I am considering of using the original one from Brooke-Gunn and Ruble (1980), but maybe there is something better? I am trying to analyze the attitude of athletes towards menstruation and its...

27 August 2020 6,541 2 View

The compensation procedure- beads (for fluorochrome) and cells (dead/live staining)- how to do it?

Hi, I would like to ask about the compensation procedure. I use Fortessa/ Diva and automated compensation procedure. I have a set from BD for compensation setting. Referring to the manufacturer...

28 February 2020 8,765 2 View

What standardised psychometric test to use for self-efficacy for female athletes?

Hello, I am looking for a valid and standardised test to measure self-efficacy of female athletes for my doctoral thesis. Any suggestions? Thank you

14 November 2019 849 3 View

What is the best recommended growth and freeze media for SH-SY5Y cells?

I received SH-SY5Y WT cells and started growing them in low glucose DMEM with FBS and S/P. After reading article about changed cell morphology and detachment of cells from growth surface due to...

06 October 2019 1,475 2 View

Why can't I load my purified pcr sample from a gel to another gel to confirm purification The product evaporates out of well before I can run the gel?

23 July 2018 3,700 8 View

Why all phages in ELISA are positive?

I perform 5 biopannings rounds and then did ELISA with the 'soup of phages' from each circle (coating with specfic antybody, then phages and antiM13). Biopannings from 1-4 had really low OD but...

18 June 2018 915 3 View

Can someone help me determine the NMR data for 2,5-dimethoxyampehtamine?

Interpretation of this compound and all the chemical shifts

20 March 2017 703 1 View

Does anybody has an experience with human serum depletion prior to MS- or electrochemical analysis?

12 March 2015 8,710 5 View

Does anyone use a thermo-desorption technique for mercury speciation in solid samples?

22 February 2015 9,352 11 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Which regression is best fit when moderator is categorical and dependent variable is latent in nature?

I am using categorical variables as a moderator variable, and my dependent, mediator and IV variables are latent in nature. Which regression test I can apply. As logistic regression is for binary...

11 February 2021 1,584 3 View

ETA-squared statistics used to correlate observed versus measured variables?

Can the eta coefficient be used when the categorical and the quantitative variable is dependent? E.g. in the field, I made a prediction into a category (e.g. high, low or medium) of what I think a...

08 February 2021 3,756 3 View

What is the best way to encode categorical variables in datasets with many features and avoid increasing dimensionality of the dataset?

I am working with a large dataset that has many categorical variables with many classes in each variable. I want to encode the categorical variables without increasing the dimensionality of the...

31 January 2021 6,986 3 View

Is it possible to compare two unequal sample sizes with categorical data on SPSS?

I have a small dataset collected from SMEs and I need to compare the demographic characteristics between SMEs with access to external finance and SMEs who do not have access to finance on SPSS....

28 January 2021 2,503 4 View

Study Design for 3 Interval DVs, mixed IVs?

I'm conducting a study on mental health terminology on social media for a group project. We have three interval DVs measured through likert scales and three IVs. The IVs are social media usage...

28 January 2021 2,871 3 View

S Is ordered logit models can applied when the DV span from negative to positive values?

Trying to understand changes in public transit use following Covid-19, our categorical DV includes 5 values: 1 and 2 indicate that participants reduced their used (to a large & moderate extent...

26 January 2021 5,918 3 View

Which statistical test is suitable to test if 2 explanatory variables (different scales: ordinal & categorical) predict dependent variable (ordinal)?

I'm working on survey data that is inclusive of ordinal (1-5 rating scale), categorical (yes/no), nominal (frequency response). I have reduced many ordinal variables (those in ordinal scale) to 3...

26 January 2021 804 12 View

Should I run an ordinal regression, or a multiple regression?

Currently I am working to defend my thesis, and I ran into a technical question while analyzing my data: Should I conduct an ordinal or a multiple regression? My model is set up such that I have...

20 January 2021 2,104 3 View

By using SPSS program, Can I do generalized structural equation modelling?

By using SPSS program, Can I do generalized structural equation modelling? All predictors are categorical data and the outcome is also categorical

14 January 2021 5,064 3 View