Hi,
Any idea on how to reduce variations among triplicates during assay such as LDH?
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I am working on cold spray technology and currently doing validation for discrete phase modelling. The total mass flow rate and initial velocity of the particles is not mentioned in the paper...
08 April 2024 9,612 4 View
I am currently validating a paper on Cold Spray technology and simulating the particle flow using 'Discrete Particle Modelling'. I got the particle tracks and the particle history data, but...
03 April 2024 9,465 2 View
I will be using the Himedia PCR cleanup protocol and kit, so if anyone has any hacks to reduce the smear, do let me know. I will be sending samples urgently for sequencing. The sequencing is...
03 January 2024 6,635 3 View
I would want to know more about the area. Any leads would be helpful. Thank you.
21 December 2023 3,500 4 View
Although there are several papers regarding EPS measurement in sludge, it is quite confusing. Like exactly what need to be measured , and how is that calculated. I would appreciate if someone...
24 November 2023 7,766 4 View
We grow the microbe in complex sugar media, spin it, take the supernatant, and add buffer and water. In the case of a test, we add the substrate and incubate it before adding DNSA, bringing it to...
05 October 2023 6,303 0 View
in terms of abroad and in India
20 August 2023 3,885 10 View
I have a hydrogel that visually looks like a gel (it is viscoelastic similar to mucus). When I do rheology on it, however it tends to show liquid-like behaviour (G''>G'), at higher angular...
17 July 2023 4,455 5 View
I need to calculate oxygen exchange current density using polarization resistance.
07 June 2023 9,237 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View