Can anyone give some idea how to reduce variations among triplicates during bioassays?

11 June 2022 0 10K Report

Hi,

Any idea on how to reduce variations among triplicates during assay such as LDH?

Badges
Science method

More Gayatri Gandhi's questions See All

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Would like to have suggestions on latest books having recent developments on Green Buildings. Can any one suggest please?

Would like to have suggestions on books I could read having recent developments in Green Buildings.

28 September 2020 2,293 7 View

How to run Seismic analysis for the following model in OpenSees..?

I have developed a 3 bay 5 story 2D frame in Opensees but I have problems with element load and application of seismic load in Opensees using an acceleration file. I always got an error namely...

27 September 2020 5,885 4 View

3D model for a nanoparticle embedded refractive index based planar waveguide?

Dear All, I kindly ask about useful references and papers related to build the 3D-geometry for a nanoparticle embedded refractive index based planar waveguides. All the relevant literature do not...

25 September 2020 9,136 2 View

How can I simulate a DOS attack in 5G?

I have been using NetSim simulator and now I want to try to simulate a DOS attack in 5G. Can someone guide me on the best way to implement this in NetSim?

27 May 2020 9,187 6 View

So suspicious, issn no: 1301-2746 (adalya journal)?

1. They claim themseleves as web of science and it is delisted from scopus sources (its been 3 years they are delisted) 2. web of science they claim annual publication (in reality they are...

27 January 2020 6,544 5 View

How do I resolve the following error I get during the execution of WRF gen_be for creating background error covariance file?

I am trying to generate the be.dat file using the gen_be facility of WRF. I have 12 hour and 24 hour outputs from WRF at 00 UTC and 12 UTC for a month. When I try to run gen_be_wrapper.ksh on my...

15 January 2020 244 2 View

Can a non-binary sensing matrix be implemented using a Digital Micromirror Device?

In compressive sensing of images Digital MicroMirror Device (DMD) is used to implement the sensing matrix. DMD works in two states, on and off. This can implement a binary sensing matrix. How is a...

05 November 2019 2,096 3 View

Embroid bodies formation from human iPSCs?

Do anyone know is it necessary to use low attachement plate instead can I use bacterological petri dish ?

10 October 2019 5,587 2 View

Can I use organisational commitment in my paper when I am only using affective commitment and normative commitment?

In my paper, I am only testing the effects of affective commitment and normative commitment but not continuous commitment. I keep having a problem in my paper when I am creating a hypothesis. Can...

18 September 2019 3,942 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View