Can anyone explain the interference from the worthington collagenase assay?

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Houda Kawas

Dear Yogi Affable

Pls. find the attached files

http://www.worthington-biochem.com/cls/assay.html

http://www.worthington-biochem.com/cls/cat.html

http://www.worthington-biochem.com/try/cat.html

http://www.worthington-biochem.com/cls/default.html

page 425 in: Hans G. Vogel, Wolfgang H. Vogel. 2013. Drug Discovery and Evaluation: Pharmacological Assays Springer Science & Business Media, 759. ISBN366203333X, 9783662033333

page 340. In: H. Gerhard Vogel. 2006.Drug Discovery and Evaluation: Safety and Pharmacokinetic Assays ; with 125 Tables Springer Science & Business Media,Pp. 889 ISBN 3540256385, 9783540256380

Nathan Back. 2012. Kinins: Pharmacodynamics and Biological Roles. Springer Science & Business Media,Pp. 398. ISBN 1468432672, 9781468432671

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1161023/pdf/biochemj00462-0106.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1166291/pdf/biochemj00584-0084.pdf

Hoping this will be helpful

Regards

Prof. Houda Kawas

Yogeswaran Jagadeesan

Thank you @ Prof. Houda Kawas..I will go through your valuable references..

You most welcome

Houda

Greetings Houda Kawas,

In the Worthigton Collagenase assay, the 25 mg of collagen should be dissolved either in 5 ml of TES buffer alone or with TES buffer contains 0.36 mM CaCl2?

Greetings Yogi Affable,

http://www.dbaitalia.it/newsletter/wbc-tg-2012-file.pdf

http://www.worthington-biochem.com/CIT/CIT_Product%20Insert_2012.pdf

http://www.worthington-biochem.com/tissuedissociation/collagenase.html

http://www.worthington-biochem.com/cls/pl.html

https://www.spandidos-publications.com/ijmm/33/3/677

https://www.researchgate.net/topic/collagenases

http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/collagenase-guide.html

Hi Prof. Houda Kawas,

I did few experiments on my collagenase enzyme.. But I getting some contradictory results.. Coould you send me you rmail ID so that I can discuss in detail

mine is [email protected]