How to perform RACE PCR

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When there are two dependent variables, should two mediation analysis be performed?

Can the first dependent variable (DV1) mediate the relationship between the IV1 and 2nd dependent variable (DV2)? IV - M1- DV1 IV- DV1(As mediator)- DV2 Can two separate mediation analysis like...

25 October 2021 1,112 2 View

A numerical integration scheme works good with structured mesh and not for unstructured mesh. What could be done to improve the accuracy ?

A integration scheme which is works good with less number of elements and fails when we increase the number of elements for the same geometric domain. What could be the reason behind that. I...

09 January 2021 4,582 3 View

Are there any ways to merge the outputs of Siamese and U Net at the end, which are trained by the same datasets?

I am training the same datasets on different networks. I want to know if there are any possibilities to combine the outputs of these two networks.

27 August 2020 6,199 1 View

Can anyone help me out with the docking procedure for a peptide sequence and protein?

Hi, I am trying to dock a peptide sequence that I generated with that of a complex protein structure. I was using chimera and Autodock Vina to do the actual docking, and It's very difficult to...

23 May 2019 320 3 View

How to isolate genomic DNA from net collected zooplankton samples for metagenomics (diveristy) studies?

Hai Anyone can explain the genomic DNA isolation methodology of net collected zooplankton samples for Metagenomics (diversity) studies. I am facing an issue in DNA isolation from net collected...

20 May 2019 6,066 4 View

Can anyone explain me how cross correlation works in pattern matching and its background process in detail?

for instance take any two images , i want to obtain correlation betweeen those two images and also i want to know how the correlation is obtained

27 November 2017 8,592 4 View

How do I calculate the recirculation length behind a bluff body in FLUENT?

Hi, I am currently working on a simulation of external flow over a semi-circular cylinder with the curved surface facing the flow. I would like to calculate the length of the recirculation region...

26 March 2017 6,877 4 View

How do I solve the divergence problem?

helloI am doing thermal and hydraulic study of flow past perforated fins using turbulent K-Omega SST model in ANSYS Fluent. After few iterations, my Energy equation start to Diverge. Please...

24 March 2017 3,397 6 View

How can I create a monitor for velocity at a point in FLUENT?

Hi, I am working on a transient simulation of the flow over a square prism and I want to create a monitor for the z-velocity at a particular point in the wake region (flow is in the +ve x...

16 February 2017 1,555 6 View

Can anyone explain the calculation part in determining the ammonia liberation in standard ammonium sulphate graph?

Hi all, I having the data related to the asparaginase enzymes. In order to calculate the enzyme activity I did few experiments.. As the result I performed standard ammonium sulphate curve and I...

02 February 2017 4,358 12 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View