Yogeswaran Jagadeesan

5 Questions 10 Answers 0 Followers

Questions related from Yogeswaran Jagadeesan

Can anyone explain the calculation part in determining the ammonia liberation in standard ammonium sulphate graph?

Hi all, I having the data related to the asparaginase enzymes. In order to calculate the enzyme activity I did few experiments.. As the result I performed standard ammonium sulphate curve and I...

03 February 2017 4,379 12 View

Can anyone explain the interference from the worthington collagenase assay?

Can anyone explain the interference from the worthington collagenase assay? Does this method reads all amino acids in the assay or the liberated amino acid from the digested collagen alone?

20 October 2016 2,391 7 View

Does anyone have a good MTT assay protocol for non-adherent cells?

I need to perform MTT assay for suspension cells (non-adherent cells). I believe the conventional MTT assay protocol wont work for suspension cells. Does anyone have a good MTT assay protocol for...

12 July 2016 3,286 3 View

How can I calculate L-asparaginase enzyme unit?

Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...

07 March 2016 918 3 View

Is there any other way to make the electrophoresed gel to react with gelatin substrate?

I forgot to add gelatin for my zymography in my gel, can I soak the gel in a solution containing gelatin (1% w/v)

18 August 2015 3,265 3 View