Yogeswaran Jagadeesan

4 Questions 6 Answers 0 Followers

Questions related from Yogeswaran Jagadeesan

Can anyone explain the calculation part in determining the ammonia liberation in standard ammonium sulphate graph?

Hi all, I having the data related to the asparaginase enzymes. In order to calculate the enzyme activity I did few experiments.. As the result I performed standard ammonium sulphate curve and I...

03 February 2017 4,341 12 View

Does anyone have a good MTT assay protocol for non-adherent cells?

I need to perform MTT assay for suspension cells (non-adherent cells). I believe the conventional MTT assay protocol wont work for suspension cells. Does anyone have a good MTT assay protocol for...

12 July 2016 3,243 3 View

How can I calculate L-asparaginase enzyme unit?

Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...

07 March 2016 855 3 View

Is there any other way to make the electrophoresed gel to react with gelatin substrate?

I forgot to add gelatin for my zymography in my gel, can I soak the gel in a solution containing gelatin (1% w/v)

18 August 2015 3,201 3 View