Can anyone explain the installation of photochemical derivatization unit for AF determination and overcoming the peaks delay in separation?

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Carmen Palermo

For a sensitive determination of aflatoxins, aflatoxin B1 and G1 must be derivatized in order to enhance the natural fluorescence. This can be accomplished with ease photochemically, by irradiation with UV light at 254 nm by inserting a device after separation on the chromatographic column.

the volume of the tube motro / UV cell gives no problems in the separation, but could improve increasing by 0.1 units the rate of flow.

Ahmed Salem Sebaei

Thanks Carmen for your replay, I got it from your paper :

(Validation of a confirmatory analytical method for the determination of aflatoxins B₁, B₂, G₁ and G₂ in foods and feed materials by HPLC with on-line photochemical derivatization and fluorescence detection) 2009.