I'm aware of:
Panexin and FastGro Synthetic, Animal Free, Chemically Defined FBS Replacement.
I'd like to supplement my standard basal media, if possible.
Actually, you could use heat-inactivated serum (Atlas Biologicals, Fort Collins, CO) and still retain the positive attributes of serum (minerals, vitamins, etc.) while denaturing the inductive / proliferative factors that are present.
Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...
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I am currently using transwell inserts with the addition of 50 uL of matrigel for an invasion assay with various osteosarcoma cell lines. I have tried various dilutions of matrigel and all result...
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25 February 2024 5,484 1 View
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15 February 2024 8,609 3 View
I need to perform a transwell assay on murine BMDMs (bone marrow derived macrophages). I am not sure how long I need to leave the macrophages in incubator to allow the migration. I am finding in...
30 January 2024 2,959 0 View
I already published an experimental research paper entitled "Neuroanthropology: Olfactory Recognition of the Self / Non-self by the Ancestral MHC: An EEG Study" in 2012 (doi:10.5539/ijb.v4n4p1)....
07 January 2024 2,508 4 View
I came across a paper on the exposure of zebrafish to BPA at realistic environmental concentrations and I found no mention of any solvent used to dissolve the BPA. Instead, the exposure was...
09 November 2023 732 1 View
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29 August 2023 3,107 10 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
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07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
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04 August 2024 1,200 2 View