Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

Only one clone of purified protein is active?

Hi! I'm wondering if anyone else has had a situation where they are purifying an enzyme, and only one clone is active? The cloned gene is genetically identical to other clones, but only this one...

18 September 2020 7,984 3 View

How can an enzyme have multiple optimal pH values?

Hi! I've been studying a particular enzyme that in in vitro experiments exhibits two optimal pH's. All other parameters are the same (incubation time, substrate, temperature, salt concentration,...

16 August 2020 6,073 10 View

Best phylogenetic analysis for a small data set?

I have a small data set consisting of 16 sequences, 919bp each. I am trying to determine the phylogenetic relationship among the individuals. I am wondering if the neighbor-joining method, maximum...

15 July 2020 3,240 8 View

(Geneious software) How do you DELETE the 'bad nucleotide' symbol of "?" from genome sequence?

Hi everyone! (I'm hoping you're all safe and healthy) I could really use some help figuring out how to remove the bad nucleotide symbol "???" in my Geneious sequence. (See attached photo) Before...

18 May 2020 7,840 1 View

What are the spectroscopic technics used in biological chemistry or biology ?

I want to kno more about the technicqs people usually use to learn how it works.

21 February 2020 6,917 2 View

How do I interpret interactions with the covariate in a repeated measures ANOVA?

I am running an analysis on repeated measures perceptual data (psychology). There are three categorical factors which specify the conditions participants viewed the stimuli in (e.g mode of...

30 January 2020 2,209 3 View

Can I perform XRD analysis on polished sample?

Dear All, I have to analyze a Co based superally sample where are present two different crystal structures of Co (the cubic and the exagonal one). I'm sure of it because I have analyzed the...

14 November 2018 3,667 3 View

Is there a product that allows mammalian cell lines to be stored at ambient temperature (18-25oC) for 96 h?

Hi I'm looking for a reagent that can be used to maintain cell viability at room temperature for at least 96 h. Does anyone have a recommendation? Thanks.

02 August 2018 8,793 4 View

SPSS:What should I do when my -2 log likelihood is really high, r squared value really low and a 0.000 result for the Hosmer-Lemeshow goodness-of-fit?

I am currently doing my masters thesis which involves data analysis using SPSS, the dataset has over 135000 items and numerous variables. I performed a test of multicollinearity for my...

22 July 2018 2,214 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View