Elisa protocol for chloroform fractions.
If you could be more specific, it would be helpful...
We are planning to quantitate poly hydroxy butyrate ( in chloroform fraction) by Elisa. Is it possible to quantitate the chloroform derived molecules by ELISA
I plan to use the kit from life technologies using CRISPR nuclease vector kit.
05 June 2014 235 1 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
I need to do a Schiff base reaction starting from a ketone and 1,10-phenanthroline-5-amino. I've seen that both of these starting materials are not very soluble in methanol and ethanol, which is...
23 June 2024 5,925 3 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
and chloroformate. But it takes 10 days to compete the reaction. How can I shorten the time of the reaction?
14 June 2024 2,924 3 View