Hello everyone, In my work I have to co culture the mesenchymal stem cells and U266 myeloma cell line, It would be very kind of you suggest me a classic protocol of that
Best regards
average particle size calculation from TEM
04 August 2024 2,921 1 View
Hi. Everyone, I am currently working on my Project about the mechanism for evaluating the information technology application ability of Australian university teachers.I wish to find out some...
20 March 2024 694 0 View
I would like to know if Australia has relevant policies or evaluation indicators for evaluating the information technology application ability of university teachers.
18 March 2024 8,610 0 View
I tried to proceed with NPT as usual in protein MD simulation in water and get the density for the whole system. However, I don't see any "density" items in my "gmx energy" selection. The same...
07 February 2024 6,848 0 View
In the co-adsorption system of graphene oxide and oxytetracycline with soil, after the adsorption equilibrium, all of the soil and a small portion of the adsorbed graphene and oxytetracycline can...
30 January 2024 719 2 View
Hey everyone, I recently conducted tensile tests on a magnesium alloy (AZ31B) using a dogbone design with a TD (90°) angle. My material was sourced as a round shelf, and I cut it to the specified...
25 January 2024 7,263 0 View
I've finished processing phosphorylation on the protein model using the PyTMs plugin in PyMOL. I've tried various methods, but I get the fatal error below on pdb2gmx. Is there a...
20 December 2023 5,396 1 View
In order to reduce the influence between domains that can cause dimerization in MD simulations through biosensor structures, FL biosensors were isolated, structural prediction was performed with...
20 December 2023 4,592 2 View
My thesis is about the inclusion of autism,now I need a tool to measure Chilidren's attitudes toward autism. I have read a paper "Another step to inclusion: Development and validation of the...
17 December 2023 6,924 1 View
Hello I am doing a research but I am not yet sure if it is correct or not. My aim is to see the relationship between the variables. IV- Mg Supplements (magnesium glycinate, lactate, and...
23 November 2023 1,229 0 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View