Can anybody recommend companies that generate custome gene knockout cell lines using CRISPR?

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Russell Linscott

Sorry we don't have an actual recommendation, but one more company that you might check with is Creative Biogene. Our listing of their service is here: http://www.linscottsdirectory.com/products/creative-biogene/Custom-Genome-Editing-Cell-Lines-4677948

Dario Pacitti

Life Technologies, Dharmacon, Sigma, Origene, System Bioscience. Agilent has a new kit that allows you to construct a custom made CRISPR vector http://www.genomics.agilent.com/en/SureGuide-Cas9-Nuclease-Kits/SureGuide-Cas9-Nuclease-Kits/?cid=AG-PT-186&tabId=AG-PR-127.