Can anybody recommend companies that generate custome gene knockout cell lines using CRISPR?

More Bing Na's questions See All

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to increase the attachment of the HEK293t cells to the glass coverslips or cell dishes?

Hey guys, I always have problems with the attachment of HEK293t cells to glass coverslips while doing IFA. They are so easy to detach from the coverslips while washing. The coverslips are already...

30 December 2020 8,381 3 View

Is this right way to get Modulation Transfer Function(MTF)?

Hi I'm trying to make a python script to get MTF curve for the input image Here is my algorithm to get MTF. 1. get Image 2. select ROI(High contrast region) 3. extract a center line out of image...

15 August 2020 6,646 3 View

Where to publish systematic review protocol?

I would like to prepare a systematic review protocol. I am wondering about any journals that will publish a protocol without charges from the authors? It does not have to be open access. The topic...

23 July 2020 4,537 8 View

Where to find recovery rate and death rate of COVID-19?

Some people, even doctors say it's not a serious disease, it's like seasonal flu, which could cause death. Where to find the recovery rate and death rate of COVID-19 in respect of older adults...

16 June 2020 6,546 12 View

How can I use with ECMWF wind and pressure in MIKE 21 HD modelling and having sinusoidal curve of surface elevation?

I have ECMWF ERA-5 wind and pressure data in NetCDF file, and i was able to convert the data to dsf2 file for use in MIKE 21 HD model. The problem that the results of the simulation (surface...

19 November 2019 3,850 3 View

How to set a Co-simulation FMU model based on Scilab/Xcos-SimulationX environment?

I am a newbie to FMU co-simulation technique, and got a few questions when trying to run my first model in Scilab/Xcos-SimulationX environment. In my understanding, the basic requirements for a...

12 September 2019 4,341 1 View

What is the solution to the error while running MaxQuant ?

Dear all, I use label-free QTOF technique (Bruker) in my project. I got a good result at the first time I analyzed .d folder using MaxQuant version 1.6.5. However, in the last 2 months, error...

18 August 2019 5,009 6 View

What is the antiviral ability of CD8 T cells at peak stage?

For example, mice are infected with acute LCMV virus. Normally, CD8 T expand and reach effector function at day 7 when virus was cleared. I am wondering what happen if mice are reinfected with...

17 August 2019 3,576 2 View

Can anyone help me to find a rapid sensitive assay for quantitative methylation analysis ?

Hello all, in my research I need to do quantitative methylation analysis on 3 candidate genes and have to do it in only 4 months, can you please help to find a fast but sensitive method for that?...

19 April 2019 7,728 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Daniel William Magomu

Hello Bing,

you can watch that video and you tell me if you like them because l prefer them.

Russell Linscott

Sorry we don't have an actual recommendation, but one more company that you might check with is Creative Biogene. Our listing of their service is here: http://www.linscottsdirectory.com/products/creative-biogene/Custom-Genome-Editing-Cell-Lines-4677948

Dario Pacitti

Life Technologies, Dharmacon, Sigma, Origene, System Bioscience. Agilent has a new kit that allows you to construct a custom made CRISPR vector http://www.genomics.agilent.com/en/SureGuide-Cas9-Nuclease-Kits/SureGuide-Cas9-Nuclease-Kits/?cid=AG-PT-186&tabId=AG-PR-127.