Dario Pacitti

3 Questions 13 Answers 0 Followers

Questions related from Dario Pacitti

IPSC differentiate after thawing, can anyone help troubleshoot please?

Dear Colleagues, I am having problems with resuscitating cryopreserved iPSCs. Upon thawing I get quite a good survival rate, but all my colonies look completely flat, without smooth borders and...

22 April 2016 4,049 6 View

Why does my cDNA prep not freeze at -20C?

Dear All, I have been preparing cDNA using Qiagen Quantitect Reverse transcription kit (cat. 20531). After completing the reaction I transferred the tubes at -20C. When I later (4 hours later)...

14 November 2015 8,774 12 View

Mitomycin C precipitates in PBS solution after storing a -20 degrees. Is this deleterious to cells/compound?

Hi everyone, I have recently purchased mitomycin C from SantaCruz to inactivate MEFs and since insufficient information was provided by the manufacturer on reconstitution and stability I had a...

28 February 2015 966 5 View