How to vary the implantation dose in simulation using SRIM software?

Hi, I am wondering if someone can direct me to an ion implantation forum My specific query is: I am learning the SRIM software for simulating ion implantation process. I am however, not finding...

05 May 2020 1,301 2 View

How to get DDSDDE for strain softening material or damage elastic material?

I am trying to implement a continuum damage mechanics based constitutive model in Abaqus using the user-defined subroutine UMAT. In that context, I am having hard time finding any available...

22 October 2019 9,426 3 View

T47D and HCC-1954 siRNA transduction using RNAiMAX(invitrogen) protocol?

Hello! I've been trying siRNA knockdown using RNAiMAX (invtrogen) but I'm not getting any significant knockdown in the T47D and HCC-1954. I'm using forward transfection. The protocol I uses is: 1....

15 March 2018 5,626 2 View

Why gold sputtering is needed before doing SEM for some polymer membrane samples?

I was making a polymer membrane for hydrogen fuel cell. in some of the papers i found people have done gold sputtering before SEM ?

05 March 2018 1,746 2 View

How to extract RNA from pseudobulb of medicinal orchids?

I am trying to extract RNA from the pseudo bulb part of a medicinal terrestrial orchid. although I got high quality RNA from leaf but while trying so from the pseudoibulb or rhizome I am facing...

27 December 2017 2,716 1 View

Will over compacting green metallic bodies (specifically BE Ti6Al4V) result in less sinterability if compared to those compacted at a lower pressure?

Working with direct powder rolled strips composed of a blend of Ti, Al and V. On sintering there is less of an increase in density of as-sintered specimens when compared to as-sintered specimens...

10 July 2017 2,743 3 View

Can someone let me know if SiC epilayers on SiC single crystals with selective doping are available commercially?

I know that doped SiC epitaxial layers on SiC single crystals are available commercially. Is it also possible to buy SiC epilayers with some regions with n-type and some regions with p-type...

26 April 2017 9,583 4 View

How to increase cormlet size of Crocus sativus (Saffron) in in vitro cultures?

One of my friend is working on this plant. Wild accesions of crocus sativus are there. however we want to increase its size for commercial exploitation.As reports on in vitro growth is quite less...

08 March 2017 8,092 3 View

Why do we have strong spin orbit coupling only the surface electrons of topological insulator not for the bulk electrons?

is it possible to have strong spin orbit coupled electronic motion in 3D??

25 October 2016 3,756 4 View

How to study phytochemistry analysis of cyanobacterial extracts?

how to study phytochemistry analysis of cyanobacterial extracts. in India are there any institute or company who can analyse the phytochemistry of extracts

20 February 2016 7,015 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Does heat in goat affected by feeding or BCS of the animal?

Does feeding affect heat in animal? if we were to add a specific feed (with data on dry matter content), how can we correlate feeding to estrus?

10 January 2021 9,719 13 View

Can you advise reference publications/standards on characterizing animal genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of animal genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of...

31 December 2020 4,924 3 View

How can i perform gene overexpression study in vivo? in sheep or mice model?

I am planning to transfect/infect a target gene directly into sheep or mice body to study its effect. need guidance please

03 December 2020 434 4 View

How to get rid of non-specific staining in IFA?

I am trying to stain Marc145 cells infected with PRRSV. For this I am using an unlabelled primary antibody against N protein of PRRSV and using Alexa 546 as secondary antibody. After staining I...

15 November 2020 1,457 5 View

Who can get this manuscript " Development of SYBR Green real-time PCR for diagnosis of fasciolosis in sheep"?

30 October 2020 9,456 2 View

Is my retinal GFAP staining accurate?

I am doing immunofluorescence staining on retinal sections from RCS rats to assess GFAP. As shown in my attached image, the GFAP staining is not consistent with most literature I've seen with...

05 October 2020 8,105 3 View