I want to immobilize the capturing antibody on a carbon working electrode using a covalent technique with EDC/NHS, but prior to that I have to introduce COOH group on the carbon electrode. So, can anyone tell me how to introduce COOH group?
I can suggest the usage of carboxylated SWCNT or MWCNT, which are commercially available
Thank you Yaroslav Korpan for your suggestion but actually carboxylated treated SWNCT and MWNCT are quite expensive and I have already purchased SPCE.
First suggestion: DOI: 10.1021/ac101519v
In this paper, the authors "anodized" graphene to introduce some oxygen groups. See "Sample Pretreatment-Graphene Anodization." in their paper. This could also work with graphite electrodes.
Second suggestion: DOI: 10.1088/0957-4484/20/37/375703
Here, oxygen groups are introduced by oxygen plasma. Should also work for graphite!
Last, there is always the option to "slightly" oxidize the electrode by an strong oxidation agent (permanganete, perchlorate - use non-aqueous environment, e.g. fumming sulfuric acid and try different times).
you can apply positive potential to your working electrode. for pencil graphite electrode, we usually apply 1.4 V for 30 s, for SPE I guess 1.6 V for 60 s is more than enought to introduce the surface with -COOH. (Reference electrode: Ag/AgCl, counter:platin, method: differential pulse voltammetry, buffer: acetate)
Good luck:)
When you find the conditions, please let us know.
You can apply 1600 mV during 180s with 0.1M H2SO4. I use this method with carbon SPE.
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