Can anybody suggest how to do Checker board assay for synergistic activity of plant extracts with antibiotics?

The activity of phytochemicals and antibiotics in combination was investigated using the checkerboard broth microdilution method. Can anybody suggest protocol for doing this as it is not clearly...

31 December 2017 5,360 6 View

Regarding analysis of RAPD gel/

Can anybody provide me information regarding software for analysis of RAPD DATA?

04 May 2017 6,741 3 View

Can any body tell what should I do with my active ethyl acetate and n-butanolic fractions of crude extracts. Should i go for LC-MS or NMR ?

I have defatted my plant material and then prepare ethanolic extract.This extract was then fractionated with Chloroform, Ethyl-acetate and n-Butanol?Then i perform anti- microbial and antioxidant...

05 June 2016 4,680 11 View

Can anybody tell me thomson reuters indexed or scopus indexed journal which publised free of cost?

i want to publish work on evaluation of in-vitro antioxidant activity of extracts and their correlation with total phenolic and flavonoid content.Please suggest some thomson reuter indexed and...

02 March 2016 3,776 1 View

How should I proceed for TLC analysis of ethyl acetate and n-butanol fractions of ethanol extract of bark?

I have done solvent -solvent fractionation of ethanolic exract of bark of my sample.Since phenolic and flavonoid content is higher in both fraction, so i want to do TLC analysis of both...

01 February 2016 5,865 8 View

Please suggest good journals with good impact factor for medicinal plants and pharmaceutical sciences?

Is there any journal which publishes free of cost in field of medicinal plants and pharmaceutical sciences?Please suggest good journals with good impact factor?

01 February 2016 9,407 0 View

Can anybody tell regarding the phenolic and flavonoid contents in fractionation process?

31 December 2015 3,673 12 View

Can somebody tell about calculation of FRAP assay of plant extract......?

i have prepare linear curve of 2.5micrmolar FeSO4 to 20 micrmolar FeSO4...also i have taken absorbance of standard Ascorbic acid and my extract at different concentration ....Then how to calculate...

11 December 2015 7,430 4 View

Suggest some good journals for my work on in-vitro antioxidant activity and its relation with phenolics and flavonoids?

I have related total phenolics, total flavonoids and antioxidant activities of extracts.Suggest some good journals of Scopus to publish my work?

11 December 2015 7,173 4 View

How can I relate FeSO4 curve with standard and extract?

11 December 2015 369 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View