Can anybody help in calculation of PIC in RAPD matrix?

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Can anybody suggest how to do Checker board assay for synergistic activity of plant extracts with antibiotics?

The activity of phytochemicals and antibiotics in combination was investigated using the checkerboard broth microdilution method. Can anybody suggest protocol for doing this as it is not clearly...

31 December 2017 5,442 6 View

Regarding analysis of RAPD gel/

Can anybody provide me information regarding software for analysis of RAPD DATA?

04 May 2017 6,833 3 View

Can any body tell what should I do with my active ethyl acetate and n-butanolic fractions of crude extracts. Should i go for LC-MS or NMR ?

I have defatted my plant material and then prepare ethanolic extract.This extract was then fractionated with Chloroform, Ethyl-acetate and n-Butanol?Then i perform anti- microbial and antioxidant...

05 June 2016 4,771 11 View

Can anybody tell me thomson reuters indexed or scopus indexed journal which publised free of cost?

i want to publish work on evaluation of in-vitro antioxidant activity of extracts and their correlation with total phenolic and flavonoid content.Please suggest some thomson reuter indexed and...

02 March 2016 3,854 1 View

How should I proceed for TLC analysis of ethyl acetate and n-butanol fractions of ethanol extract of bark?

I have done solvent -solvent fractionation of ethanolic exract of bark of my sample.Since phenolic and flavonoid content is higher in both fraction, so i want to do TLC analysis of both...

01 February 2016 5,952 8 View

Please suggest good journals with good impact factor for medicinal plants and pharmaceutical sciences?

Is there any journal which publishes free of cost in field of medicinal plants and pharmaceutical sciences?Please suggest good journals with good impact factor?

01 February 2016 9,517 0 View

Can anybody tell regarding the phenolic and flavonoid contents in fractionation process?

I have defatted my extract and then prepare ethanolic extract....after that I dissolved my extract in water and then fractionate firstly with chloroform, ethyl acetate and then with n-butanol...

31 December 2015 3,762 12 View

Can somebody tell about calculation of FRAP assay of plant extract......?

i have prepare linear curve of 2.5micrmolar FeSO4 to 20 micrmolar FeSO4...also i have taken absorbance of standard Ascorbic acid and my extract at different concentration ....Then how to calculate...

11 December 2015 7,522 4 View

Suggest some good journals for my work on in-vitro antioxidant activity and its relation with phenolics and flavonoids?

I have related total phenolics, total flavonoids and antioxidant activities of extracts.Suggest some good journals of Scopus to publish my work?

11 December 2015 7,253 4 View

How can I relate FeSO4 curve with standard and extract?

I hav taken absorbance of FESO4 at range of 2.5-200 uM which show linearity with R2=O,98...then i took absorbance of standard/extract at different conc .....how to get FRAP activity...show me...

11 December 2015 460 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View