Can anybody suggest how to do Checker board assay for synergistic activity of plant extracts with antibiotics?

The activity of phytochemicals and antibiotics in combination was investigated using the checkerboard broth microdilution method. Can anybody suggest protocol for doing this as it is not clearly...

31 December 2017 5,360 6 View

Can anybody help in calculation of PIC in RAPD matrix?

I am unable to calculate PIC value of primers in RAPD.Can some one help in calculating PIC stepwise? An example of one primer in excel sheet is attached.

07 August 2017 9,708 2 View

Regarding analysis of RAPD gel/

Can anybody provide me information regarding software for analysis of RAPD DATA?

04 May 2017 6,741 3 View

Can any body tell what should I do with my active ethyl acetate and n-butanolic fractions of crude extracts. Should i go for LC-MS or NMR ?

I have defatted my plant material and then prepare ethanolic extract.This extract was then fractionated with Chloroform, Ethyl-acetate and n-Butanol?Then i perform anti- microbial and antioxidant...

05 June 2016 4,680 11 View

Can anybody tell me thomson reuters indexed or scopus indexed journal which publised free of cost?

i want to publish work on evaluation of in-vitro antioxidant activity of extracts and their correlation with total phenolic and flavonoid content.Please suggest some thomson reuter indexed and...

02 March 2016 3,776 1 View

Please suggest good journals with good impact factor for medicinal plants and pharmaceutical sciences?

Is there any journal which publishes free of cost in field of medicinal plants and pharmaceutical sciences?Please suggest good journals with good impact factor?

01 February 2016 9,407 0 View

Can anybody tell regarding the phenolic and flavonoid contents in fractionation process?

31 December 2015 3,673 12 View

Can somebody tell about calculation of FRAP assay of plant extract......?

i have prepare linear curve of 2.5micrmolar FeSO4 to 20 micrmolar FeSO4...also i have taken absorbance of standard Ascorbic acid and my extract at different concentration ....Then how to calculate...

11 December 2015 7,430 4 View

Suggest some good journals for my work on in-vitro antioxidant activity and its relation with phenolics and flavonoids?

I have related total phenolics, total flavonoids and antioxidant activities of extracts.Suggest some good journals of Scopus to publish my work?

11 December 2015 7,173 4 View

How can I relate FeSO4 curve with standard and extract?

11 December 2015 369 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View