Can anybody suggest how to do Checker board assay for synergistic activity of plant extracts with antibiotics?

The activity of phytochemicals and antibiotics in combination was investigated using the checkerboard broth microdilution method. Can anybody suggest protocol for doing this as it is not clearly...

31 December 2017 5,360 6 View

Can anybody help in calculation of PIC in RAPD matrix?

I am unable to calculate PIC value of primers in RAPD.Can some one help in calculating PIC stepwise? An example of one primer in excel sheet is attached.

07 August 2017 9,708 2 View

Can any body tell what should I do with my active ethyl acetate and n-butanolic fractions of crude extracts. Should i go for LC-MS or NMR ?

I have defatted my plant material and then prepare ethanolic extract.This extract was then fractionated with Chloroform, Ethyl-acetate and n-Butanol?Then i perform anti- microbial and antioxidant...

05 June 2016 4,680 11 View

Can anybody tell me thomson reuters indexed or scopus indexed journal which publised free of cost?

i want to publish work on evaluation of in-vitro antioxidant activity of extracts and their correlation with total phenolic and flavonoid content.Please suggest some thomson reuter indexed and...

02 March 2016 3,776 1 View

How should I proceed for TLC analysis of ethyl acetate and n-butanol fractions of ethanol extract of bark?

I have done solvent -solvent fractionation of ethanolic exract of bark of my sample.Since phenolic and flavonoid content is higher in both fraction, so i want to do TLC analysis of both...

01 February 2016 5,865 8 View

Please suggest good journals with good impact factor for medicinal plants and pharmaceutical sciences?

Is there any journal which publishes free of cost in field of medicinal plants and pharmaceutical sciences?Please suggest good journals with good impact factor?

01 February 2016 9,407 0 View

Can anybody tell regarding the phenolic and flavonoid contents in fractionation process?

31 December 2015 3,673 12 View

Can somebody tell about calculation of FRAP assay of plant extract......?

i have prepare linear curve of 2.5micrmolar FeSO4 to 20 micrmolar FeSO4...also i have taken absorbance of standard Ascorbic acid and my extract at different concentration ....Then how to calculate...

11 December 2015 7,430 4 View

Suggest some good journals for my work on in-vitro antioxidant activity and its relation with phenolics and flavonoids?

I have related total phenolics, total flavonoids and antioxidant activities of extracts.Suggest some good journals of Scopus to publish my work?

11 December 2015 7,173 4 View

How can I relate FeSO4 curve with standard and extract?

11 December 2015 369 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View