Regarding analysis of RAPD gel/

More Vikas Kumar's questions See All

Can anybody suggest how to do Checker board assay for synergistic activity of plant extracts with antibiotics?

The activity of phytochemicals and antibiotics in combination was investigated using the checkerboard broth microdilution method. Can anybody suggest protocol for doing this as it is not clearly...

31 December 2017 5,442 6 View

Can anybody help in calculation of PIC in RAPD matrix?

I am unable to calculate PIC value of primers in RAPD.Can some one help in calculating PIC stepwise? An example of one primer in excel sheet is attached.

07 August 2017 9,796 2 View

Can any body tell what should I do with my active ethyl acetate and n-butanolic fractions of crude extracts. Should i go for LC-MS or NMR ?

I have defatted my plant material and then prepare ethanolic extract.This extract was then fractionated with Chloroform, Ethyl-acetate and n-Butanol?Then i perform anti- microbial and antioxidant...

05 June 2016 4,771 11 View

Can anybody tell me thomson reuters indexed or scopus indexed journal which publised free of cost?

i want to publish work on evaluation of in-vitro antioxidant activity of extracts and their correlation with total phenolic and flavonoid content.Please suggest some thomson reuter indexed and...

02 March 2016 3,854 1 View

How should I proceed for TLC analysis of ethyl acetate and n-butanol fractions of ethanol extract of bark?

I have done solvent -solvent fractionation of ethanolic exract of bark of my sample.Since phenolic and flavonoid content is higher in both fraction, so i want to do TLC analysis of both...

01 February 2016 5,952 8 View

Please suggest good journals with good impact factor for medicinal plants and pharmaceutical sciences?

Is there any journal which publishes free of cost in field of medicinal plants and pharmaceutical sciences?Please suggest good journals with good impact factor?

01 February 2016 9,517 0 View

Can anybody tell regarding the phenolic and flavonoid contents in fractionation process?

I have defatted my extract and then prepare ethanolic extract....after that I dissolved my extract in water and then fractionate firstly with chloroform, ethyl acetate and then with n-butanol...

31 December 2015 3,762 12 View

Can somebody tell about calculation of FRAP assay of plant extract......?

i have prepare linear curve of 2.5micrmolar FeSO4 to 20 micrmolar FeSO4...also i have taken absorbance of standard Ascorbic acid and my extract at different concentration ....Then how to calculate...

11 December 2015 7,522 4 View

Suggest some good journals for my work on in-vitro antioxidant activity and its relation with phenolics and flavonoids?

I have related total phenolics, total flavonoids and antioxidant activities of extracts.Suggest some good journals of Scopus to publish my work?

11 December 2015 7,253 4 View

How can I relate FeSO4 curve with standard and extract?

I hav taken absorbance of FESO4 at range of 2.5-200 uM which show linearity with R2=O,98...then i took absorbance of standard/extract at different conc .....how to get FRAP activity...show me...

11 December 2015 460 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

Can anyone help in getting software related to gel scoring of silver stained PAGE for diversity study?

I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...

12 July 2024 7,145 2 View

Subramani Pandian

for genetic diversity analysis.....? if yes please find the attachment,

you can use STRUCTURE Software for population structure analysis...

DARWin, Popgene, NTSYS pc version etc

Vikas Kumar

Thanks a lot sir...But i have done only RAPD.....

Seied Mehdi Miri

Dear Vikas

You can also see the following link for more information.

https://www.google.com/amp/s/www.researchgate.net/post/Best_software_tools_for_the_analysis_of_AFLP_and_RAPD_dataset2/amp