Prostate cancer cells PC-3 and LNCaP show differential sensitivity. PC-3 is least sensitive to androgen whereas LNCaP cells show maximum sensitivity to androgens..
MCF-7(Estrogen sensitive) and MDA-MB-231 (Least sensitive)breast cancer cells also display differential sensitivity...
I agree with Sridhar, MCF-7 are Estrogen Receptor positive, in contrast MDA-MB are Estrogen Receptor negative.
Positive for hormone receptors does not necessarily mean responsive (receptor may not function). For few breast cancer cell lines, status for estrogen, insulin and IGF1 are given in the attached slide (from Archives of Physiology and Biochemistry 03/2008; 114(1):38-44. doubleclick for better magnification).
I can confirm what has been reported by colleagues. In addition, I would like to add that even DU145 cell lines are hormone-refractory prostate cancer cells (as the PC3) in comparison to LnCAP which are hormone-sensitive prostate cancer cells. Furthermore, the following work could be useful http://link.springer.com/article/10.1007%2FBF00419290
I have worked on both the types of prostate cancer cell lines. Androgen receptor (AR) dependent and non dependent also. It is known that PC3 and DU145 are AR independent and LNCaP is AR responsive cell line. But androgen dependency has been a matter of doubt as they express AR protein. Please go through the following text:
The majority of human prostate cancer cell lines, including the two “classical” cell lines DU-145 and PC-3, are reported to be androgen receptor (AR)-negative. However, other studies have provided evidence that the DU-145 and PC-3 cell lines express AR mRNA. It was reported that DU-145 and PC-3 cell lines express AR protein and it was found that the relative levels of the AR mRNA and protein that were detected in DU-145 and PC-3 cell lines were lower than the LNCaP, an AR-positive cell line. Moreover, the antibody directed against the non-variant region (amino acids 299–315), but not the variant N- or C-terminal region (amino acids 1–20 and 900–919, respectively) of the human AR protein, detected the expression of AR in all prostate cancer cell lines. Notably, treatment of these cell lines with dihydrotestosterone (DHT) resulted in measurable increases in the AR protein levels and considerable nuclear accumulation. Although, treatment of DU-145 and PC-3 cells with DHT did not result in stimulation of the activity of an AR-responsive reporter, knockdown of AR expression in PC-3 cells resulted in decreases in p21CIP1 protein levels, and a measurable decrease in the activity of the p21-luc-reporter. These observations demonstrate the expression of AR protein in DU-145 and PC-3 prostate cancer cell lines (Alimirah et al., 2006).
Reference:
Alimirah F, Chen J, Basrawala Z, Xin H, Choubey D. DU-145 and PC-3 human prostate cancer cell lines express androgen receptor: Implications for the androgen receptor functions and regulation. FEBS Lett 2006, 580, 2294-2300.
yes i agree with Alok sir's point... PC-3 is very less sensitive to hormones and most of the literature stating that PC-3 cell lines are AI (Androgen Independent).. that's why hormone treatment fails with some prostate cancer patients.. and if you are working on PC-3 cell lines, try to identify biomarkers that can help to discover a new drug which can possibly decrease the metastasis in prostate cancer... All the best...
Prostate cancer cells PC-3 and LNCaP show differential sensitivity in cytotoxicity tests. LNCAP is more sensitive than PC3 cells
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