Priyanka Srivastava

10 Questions 20 Answers 0 Followers

Questions related from Priyanka Srivastava

DSMZ 120a medium precipitation after autoclave?

Hi, I am trying to prepare DSMZ 120a for methanosarcina according to the mentioned protocol on DSMZ site. But everytime I autoclave, I see the precipitation of medium. The pH of media was 6.8. Can...

15 May 2019 9,737 1 View

Preparation of L-Cysteine-HCl x H2O for M.barkeri?

Hi, I have to prepare L-cysteine solution in HCl and water but don't know the recipe. Can anyone please suggest me the protocol to prepare this solution.

28 March 2019 9,743 5 View

Anaerobic serum bottles pressure range?

Does anyone know the range of pressure that 120ml anaerobic serum bottles can tolerate (10-100 psi)?

27 February 2019 7,087 3 View

Isolation and identification of proteins?

I am looking for the protein expression of Methanosarcina mazeii that can get expressed in the presence of acetate. I am not much aware of steps. I was planning to do cell-lysis and then SDS-PAGE...

21 February 2019 8,399 11 View

White things present in anaerobic culture bottle with coal powder as a substrate?

I am getting a white crystalline things in my anaerobic culture bottles. I have used same medium from a year and this never happened before. Can anyone suggest me, what this could be? Just for...

14 November 2018 1,838 1 View

Is there any standard or level, that can decide the maximum dose, to be given to the cancer patient that won't make cancer cells resistant?

I have worked on therapeutic approaches against breast, prostate and brain carcinoma. Nowadays, the main issues this world is facing, is cancer cells getting resistant to treatment/therapeutics...

29 December 2014 8,376 17 View

How can I extract integral membrane protein from BL-21 cells?

I have tried sonication, but sonicator is not working now, so what reagent should I use to take out the integral protein. I have tried freeze thawing by using liquid nitrogen and 42 C temperature,...

31 October 2014 9,510 5 View

What are the ways to use thymoquinone in animals for breast cancer studies?

I have achieved 90 % killing in-vitro on MCF-7 and now I am proceeding in-vivo. I will be using BALB c mice for studies. I will inject 4T1 (mouse myeloma) that mimics breast cancer. What are the...

20 August 2013 3,758 1 View

Can someone advise on using animal models for prostate cancer?

Can we use normal mice for study of prostate cancer? And what other animal models can be used for prostate cancer as well as breast cancer.?

27 July 2013 4,829 2 View

Can any one tell me about carcinoma cell lines that are hormone sensitive as well as hormone resistant cell lines?

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15 June 2013 5,358 8 View