Can any one suggested possible way to measure ATP level in cell ?

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Amado Carreras-Sureda

Hi I did luciferase assay normalized to protein levels. (Sigma)

Also there are ATP FRET proves tagged to cytosol and mitochondria, they work wuite well!

Hope this helps!

Agnieszka Bojko

I was using this kit to measure it, simple and easy:

https://www.perkinelmer.com/lab-solutions/resources/docs/MAN-ATPlite.pdf

Good luck!

Michael Koksharov

Apart from the enzymatic assay (beetle luciferase), you can use at least the following:

1) NMR (works in live samples)

2) HPLC

3) LC-MS

4) molecular sensors in live cells (based on fluorescent proteins, luciferases, fluorescent dyes)

In all destructive methods beware of the pitfalls (easy ATP degradation during the sample preparation):

https://www.researchgate.net/post/Which_is_a_good_Tissue_Cell_Lysis_Buffer_for_Luminescent_ATP_assay

Mamunur Rashid

Dear Michael

I am extremely thankful to you for your valuable suggestion. can you link paper where ATP were estimated by HPLC or LC-MS. Actually i want to know extraction procedure how it could be estimated by HPLC or LC-MS.

2) HPLC:

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3) LC-MS:

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Thanks Michael for kindness