If you plan on using this media in a cell culture incubator (i.e. 5-10% CO2) then absolutely DO NOT use D-PBS or HBSS (as in Hanks balanced salt solution). Phosphate buffer does not buffer well against CO2 and your pH will drop to less than 6.5 within 30 minutes. EBSS would be a good choice in that case since it is buffered with bicarbonate, and happens to be the base for MEM/DMEM. If you plan on doing the experiment at room atmosphere, then D-PBS or HBSS should be fine. Just be sure to supplement back in glucose (presumably at the same concentration the cells were in originally), unless you want to completely nutrient deprive your cells. Short term incubation (2-4 hours) in AA free medium does not require the addition of vitamins. In terms of using serum, you can leave the medium serum free or use dialyzed serum, depending on the purpose of your experiment.
You can just prepare it yourself. DMEM without amino acids is just a salt/sugar/vitamin solution. You can look up the formulation of the media you're interested in and mix your own.
Actually I read in papers that using D-PBS we can deprive amino acids. So i want to plan in that way.
If you plan on using this media in a cell culture incubator (i.e. 5-10% CO2) then absolutely DO NOT use D-PBS or HBSS (as in Hanks balanced salt solution). Phosphate buffer does not buffer well against CO2 and your pH will drop to less than 6.5 within 30 minutes. EBSS would be a good choice in that case since it is buffered with bicarbonate, and happens to be the base for MEM/DMEM. If you plan on doing the experiment at room atmosphere, then D-PBS or HBSS should be fine. Just be sure to supplement back in glucose (presumably at the same concentration the cells were in originally), unless you want to completely nutrient deprive your cells. Short term incubation (2-4 hours) in AA free medium does not require the addition of vitamins. In terms of using serum, you can leave the medium serum free or use dialyzed serum, depending on the purpose of your experiment.
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