Can anyone tell me how to design primers for the known promoter region for ChIP?

More Praveen kumar patnaik's questions See All

Can any one suggest me single copy or very low copy shuttle vectors (bacteria/yeast) ?

Dear all RG members, We are working on a project which deals with the functionality of one of the genes in yeast. We wanted to transform a single copy plasmid containing our gene of interest into...

10 November 2017 942 2 View

How to ligate incompatible overhangs ?

I would like to know is it possible to ligate incompatible overhangs formed at 5/ and 3/ end of plasmid.

04 May 2017 4,690 7 View

Can any one suggest how to deprive total amino acids from culture medium (DMEM)?

See above

07 August 2014 731 5 View

How is metastasis specific?

Prostate tumors are most often metastases to the bones, while colon carcinomas preferentially form new colonies in the liver. Is there any known mechanism for this specific trans-location of the...

04 May 2014 1,265 3 View

How do cancer cells learn to become metastatic?

Metastases are formed by cancer cells that have left the primary tumor mass and traveled by the body’s highways—blood and lymphatic vessels— to seek out new sites throughout the body where they...

04 May 2014 761 8 View

Being a master regulator what is the role of neuronal signaling in controlling metastasis?

Is there any role of master regulator i.e Brain in metastasis of primary tumor?

04 May 2014 5,547 1 View

How can I know the origin of the metastasized cell?

How can we understand the primary habitat of a metastasized cell?

03 April 2014 2,091 7 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Sandeep Rajput

Check literature for know binding sites of your transcription factor on promoter region. Based on that design your primers for CHIP assay. And if nothing is known about transcription factor and its binding site, go for CHIP sequencing assay. Qiagen provides kits for CHIP sequencing assay.

Praveen kumar patnaik

But chip seq is quite expensive..... So I want to go with the random primers.. Is there any bioinformatic tool to know the specific protein binding site on promoter???

genomatix software. You can also check on ebi.ac.uk website.