We want an efficient assay for detection of precursor and mature micro RNA.Can any one suggest some kits or manual methods?
Dear Nitika,
Please see the following:
https://www.thermofisher.com/order/catalog/product/AM1552
https://www.thermofisher.com/order/catalog/product/AM1558
https://www.thermofisher.com/order/catalog/en/US/adirect/lt?cmd=catDisplayStyle&catKey=18101&filterDispName=miRNA%2BqRT-PCR%2BKits&_bcs_=H4sIAAAAAAAAAMVPwWrDMAz9Gl9mUpwY2lyzdh2jMEra9W5stTHYcbCVhfz95JVCD2X0NhB6lvTw%0Ae68omaj3MZhRY%2BKsWvIDxG%2BrIf2x7xAHJhtWbammaVpgB9GHs02ECx08rcdEDXpqXfBAEKKhY4fe%0A0Q%2BskrlEjXGEPIuVIChFScy6EuULTTt7lVae1F55k5Kan5OXTS1ukoRaoXLhcjP0daCmjI2gkV4O%0Amdxqb5jcfJzeP4m8sWlwal4rhEuIM1mg5Q5mYmRv9%2F7PyqWHAQjK1fIapAXliqP1wPfr9i7RW2%2BG%0AYHvk7bHIlxz4n%2FP9en4U8AetOx54KQIAAA%3D%3D
http://www.genomics.agilent.com/en/Specialty-Master-Mixes-Kits/miRNA-QRT-PCR-Detection-Kit/?cid=AG-PT-176&tabId=AG-PR-1109
http://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/mirna-ncrna-taqman-assays/taqman-advanced-mirna-assays.html?gclid=CL2esJq-qsoCFROeGwodS9kLTA&s_kwcid=AL!3652!3!73642342692!b!!g!!microrna%20profiling&ef_id=UWQUhgAAAHpimj79:20160114234556:s
https://www.google.co.il/?gfe_rd=cr&ei=0NyXVtrkOKih8wf4sIOABA&gws_rd=ssl#
Hoping this will be helpful,
Rafik
please see the following links:
http://www.ncbi.nlm.nih.gov/pubmed/25574760
http://www.ncbi.nlm.nih.gov/pubmed/19367400
https://www.thermofisher.com/in/en/home/references/ambion-tech-support/microrna-studies/tech-notes/detecting-mirna-and-sirna-.html
https://www.thermofisher.com/in/en/home/references/ambion-tech-support/microrna-studies/tech-notes/detect-and-quantitate-microrna-in-laser-capture-microdissection-.html
http://www.nature.com/nmeth/journal/v3/n1/full/nmeth0106-12.html
Hope these articles and links will be helpful for you..!!!
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