Actually, I need this thermal cycler device in my lab. But the faculty budget for the devices this year and the next year is finished. Some colleagues advised me to search for other universities that have several used devices to give one to my faculty as a gift from university to a university
Might be worth it to check out this web site of equipment donation program:
http://seedinglabs.org/
If any one accepted to give you a gift, make sure that electricity of thermal cycler is 220 volts not 110 volts
Might be worth it to check out this web site of equipment donation program:
http://seedinglabs.org/
Your colleagues are right. A lot of labs, particularly in microbial diagnostics, use only real time PCR now, and they are happy to get rid of their conventional PCR machines. However, the old machines do tend to be quite heavy and bulky, so the price of shipping may be more than the cost of a small modern instrument.
I would guess that if you sent an e-mail to all the medical diagnostic laboratories in Scandinavia, your chances of getting a free PCR machine would be nearly 100%.
And if all else fails, it is perfectly possible to do PCR with three thermostatted waterbaths, a rack of tubes and a timer (you move your tubes to the next bath at the end of each PCR stage). I did this for about a year before we got our first PCR machine. It's very boring, but it works surprisingly well.
thanks Dr/ Andrew Jenkins. i will do the two options but could you kindly explain for me the typical conditions for the second option (three thermostatted waterbaths). i understood that i will prepare 3 water bathes one for denaturation and one for annealing and one for elongation and i will move the tube rack between the 3 water bathes for 35 cycle as an example but i want to know how i can keep the temp while moving form water bath to another and what about the final elongation. please give me more details. thanks again for your advice
Hei Noha,
The question of maintaining the temperature while moving from one waterbath to another is not an issue as you will need to have them right next to each other; you can move your rack of tubes in a second or less, so they don't have time to air-cool.
If you want to do a final elongation step, just leave the tubes in the 72 C bath for ten minutes.
Hello Noha,
I just wanted to find out if you eventually got someone to donate the thermal cycler you needed.
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