We want to use F2:3 chilli population for QTl identification. But how to take phenotype data and genotype segregation data
Better you developed RILs with these lines and then did QTL analysis, Regarding Phenotype, Gerrnally it depned on your trait of interest, and the parents of this mapping pupolation should be contrasting.
In genotyping, you should select polymorpic markers, and run on your mapping population and make a linkage map and then done QTL mapping using softwares (RQTLs, QTL carto, ICIM etc)
I am doing QTL mapping for plant height and other traits in F2 populations, I feel following consequences
1. Population size is very less
2. You cannot take data in replications , its possible in RILS
Better advance the generation as RILS
BEST
The NC III design is better than F2 alone. And RILs are made too long, DH are faster.
SR KRISHNA MOTUKURI Please refer to Article An introduction to markers, quantitative trait loci (QTL) ma...
Msr Krishna go through this paper.
Collard, B.C.Y., Jahufer, M.Z.Z., Brouwer, J.B. et al. Euphytica (2005) 142: 169. https://doi.org/10.1007/s10681-005-1681-5
It will be cleared your all doubt.
From consider all population for phenotype data as well as use for molecular analysis
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