i want to extract RNA from the cornea. kindly suggest me a simple method for frozen cornea.
Hi :)
I ever did RNA extraction for tissue even it was not cornea, but i think this way might be possible for your tissue sample. I used mortar and pestle. Put your enough tissue sample inside the mortar (if it is possible, cut your tissue into small pieces at the beginning), then pour enough liquid nitrogen and mash it while it's immersed in liquid nitrogen. At the end, that's suppose to be dried and you will get powder-like sample. Put it in eppendorf or any processing tube. After that you can do the RNA extraction by following the protocol that you use.
I hope this sharing could be helpful for your experiment. Good luck!
Please check tissue crushers
https://homogenizers.net/products/biopulverizer?utm_medium=cpc&utm_source=googlepla&gclid=CILX3fmDpNICFdcegQodLAEHIQ
You also may try to cut (finely) your tissues with sharp scissors into a tube with Qiazol and homogenize it with beads or pestles, for example
http://www.eandkscientific.com/1.5ml-Homogenization-Pestle.html.
Good luck!
Yes you can sure frozen sample for extraction but for better homogenise efficiently
Use liquid nitrogen and pulverize your sample. Pour in your mortar & pestle and grind. It can be done with glass as well (pyrex I believe) Check it w/o sample first.
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