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Questions related from Kellie Bowen
I'm willing to do fluorescence or DAB. Rat muscle, snap frozen, 10um cryostat sections.
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I'm looking for alpha synuclein aggregates. I have a few questions about the technique.
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I generally flush the rat with saline before harvesting tissues for western blots. I have heard that the blood and other components can interfere with blots. Does anyone else flush the animal...
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Also a good protocol for IHC with it.
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