Can any body help me for identification of this species?

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Exact mechanism of anti-cancer agents.?

What is the exact mechanism of synthetic agents showed the cell cycle arrest in G0/G1 and G2/M2 phase in flow cytometry analysis.

28 June 2024 5,950 2 View

Curcumin encapsulation in Bovine Serum albumin (BSA)?

Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...

23 June 2024 8,785 5 View

How can I implement GAFF-LCFF forcefield in GROMACS?

Dear experts, I am new to molecular dynamics and can run simulations on small Liquid crystal systems using GAFF in GROMCS. I wanted to use the GAFF-LCFF forcefield (Optimization of the GAFF Force...

11 June 2024 4,547 2 View

Albumin Nanoparticle Synthesis, troubleshooting?

Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...

04 June 2024 1,501 0 View

How to publish a paper in the journal "CELL" ?

Is Cell the unit of living organism / life? Absolutely incorrect. Explanation: From fossils cell we have estimated , before how many years it was there, e.g 40millions years etc.. Was that cell...

28 May 2024 5,931 1 View

How to get 2 Octanol from Castor oil at a laboratory scale ?

Any latest method developed

24 May 2024 2,128 1 View

Uncallable SNP showed significant to MTA, what to do?

I am getting a significant marker trait association for traits. But understanding the allelic association is difficult; NN is the missing allele. Can anyone help me?

13 May 2024 8,860 2 View

What are the different ways to apply the boundary conditions for a compact tension (CT) specimen in FEM modelling.?

Can we restrict the vertical and horizontal displacement in two directions for the lower semicircular in bottom hole of CT specimen and apply incremental displacement in the upper semicircular in...

09 May 2024 5,273 4 View

Difficulty in Phase separation ?

Hey all I intend to isolate free circulating SiRNA in media using chloroform extraction. I have added chloroform to media containing SiRNA to get the phase separated. The Chloroform came in the...

17 April 2024 8,522 3 View

Why negative dielectric loss is coming at lower frequency range i.e from 4 to ~100 Hz?

I have performed the temperature dependent dielectric measurements;with proper checking of all electrical connections of electrodes, sensors,heater etc.as well as their proper resistance of...

07 April 2024 7,625 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View