Acids & Bases Buffers Deprotonation
Yes. You just need the pH of the buffer to be higher than the pKa of the compound.
The characteristic raman spectra that I've obtained for graphene like quantum dots shows a kink at about 1450 cm-1, this is different from the reported literature. What can be the posible reason...
01 February 2020 3,135 3 View
I've a nano-scaled graphite like material, the XRD pattern is showing some peaks which are not matching with any of the reported literature. For example usually a broad peak is seen around 21...
01 February 2020 6,479 2 View
I have to analyse the change in composition of acetonitrile after being reacted mechanically at different time intervals, hence I'm planning to undertake a GC-MS of the samples. Can anyone suggest...
05 June 2019 9,342 8 View
What kind of synthesis is this SSM route useful for?
03 April 2018 10,226 2 View
I'm using a PDA detector HPLC unit, I was wondering whether it is possible that the retention time can change for a particular compound when the detector is set at a different wavelength, all...
11 December 2017 5,613 6 View
I'm withdrawing samples after a 15 minute interval for 90 minutes. Hence, the 90th minute sample is to show the highest amount of degradation or the least peak area, but it shows the highest, this...
10 November 2017 6,053 7 View
How to identify crystalline phase data from the XRD data of the said crystals
08 September 2017 591 2 View
In most research papers the wavelength chosen for DBT detection is 284nm, but according to the NIST data the maximum absorbance occurs around 230-240nm. Can anyone explain why it is...
07 August 2017 4,252 4 View
How do I choose the best conditions for this sort of HPLC?
07 August 2017 8,424 4 View
statistical analysis, software, data analysis designing experiments
07 August 2017 5,929 4 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View