Different Raman spectra of graphene like quantum dots?

The characteristic raman spectra that I've obtained for graphene like quantum dots shows a kink at about 1450 cm-1, this is different from the reported literature. What can be the posible reason...

01 February 2020 2,243 3 View

How can I index XRD peaks of a graphite like sample?

I've a nano-scaled graphite like material, the XRD pattern is showing some peaks which are not matching with any of the reported literature. For example usually a broad peak is seen around 21...

01 February 2020 6,185 2 View

What can be the perfect GC-MS operating conditions for acetonitrile?

I have to analyse the change in composition of acetonitrile after being reacted mechanically at different time intervals, hence I'm planning to undertake a GC-MS of the samples. Can anyone suggest...

05 June 2019 9,128 8 View

What is a Solid State Metathetic Reaction?

What kind of synthesis is this SSM route useful for?

03 April 2018 9,998 2 View

Can retention time for a particular compound change when detected at various wavelengths?

I'm using a PDA detector HPLC unit, I was wondering whether it is possible that the retention time can change for a particular compound when the detector is set at a different wavelength, all...

11 December 2017 5,337 6 View

What can be the plausible reason for increase in the area of a peak in HPLC spectrum, even when the reaction is to degrade the compound?

I'm withdrawing samples after a 15 minute interval for 90 minutes. Hence, the 90th minute sample is to show the highest amount of degradation or the least peak area, but it shows the highest, this...

10 November 2017 5,853 7 View

What is the JCPDS card number for potassium ferrioxalate trihydrate crystals?

How to identify crystalline phase data from the XRD data of the said crystals

08 September 2017 345 2 View

Why is the lambda max of dibenzothiophene chosen as 275-284nm and not 230-240nm?

In most research papers the wavelength chosen for DBT detection is 284nm, but according to the NIST data the maximum absorbance occurs around 230-240nm. Can anyone explain why it is...

07 August 2017 3,923 4 View

What could be the HPLC conditions for thianaphthene/benzothiophene?

07 August 2017 8,177 4 View

Which is the better analytical software Modde Pro or Design expert? Why?

07 August 2017 5,576 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View