I would like to study the dehydration of carbohydrates to HMF in other solvents than water, however I am not too sure if the HPLC column that we have is appropriate for analysing this type of solutions.
https://www.bgb-info.com/home.php?cat=28429
https://www.hplc.eu/Downloads/Alltech_IC_Columns.pdf
Thank you for the links ( I have had already seen them before), but unfortunately they do not answer my question if the solvent of the sample could/could can not be organic... Anybody has any experience using this column for solutes in organic solvent not aqueous?
Thanks for the answers!
It says clearly no organic solvents as mobile phase, but they do not mention anything about the solvent in the sample which is in much lower quantity. I inject around 20 uL. I have contacted the technical support from the column supplier and this is what they said:
Thanks you for your enquiry. I've looked into the solvent compatibility for you and this is the information I've got.
"DMSO as a cleaning solvent is not good. As a component in a 20uL sample, even along with MIK, will be fine. We don't recommend 100% DMSO...diluted in a sample is fine."
I also discussed the use of alcohols and MIBK with my colleagues when I looked into solvent compatibility and there was no mention of either being an issue. I assumed from your email the solvents you detailed will only be used in sample preparation, so they will be present in small amounts. I would try to minimise the use of DMSO as much as possible, keeping it in as dilute as you can in your sample.
So now based on what they say and what u say I am still not sure what to do??
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