Hi,
I would like to ask whether a silenced/knockdown gene mediated with siRNA can be reactivated/restored? I have knockdowned Nrf2 transcription factor (a master regulator of oxidative stress) with 70% , and we see upregulation of Nrf2 and its target genes after treatment of a conditioned media (derived from supernatant of a cell line).
Is it possible to observe reactivation/restoration? Anyone experience this before and got any papers showing or papers related to Nrf2?
Hope to hear from you all. Thanks