Hi,

I would like to ask whether a silenced/knockdown gene mediated with siRNA can be reactivated/restored? I have knockdowned Nrf2 transcription factor (a master regulator of oxidative stress) with 70% , and we see upregulation of Nrf2 and its target genes after treatment of a conditioned media (derived from supernatant of a cell line).

Is it possible to observe reactivation/restoration? Anyone experience this before and got any papers showing or papers related to Nrf2?

Hope to hear from you all. Thanks

More Yuan-Seng Wu's questions See All
Similar questions and discussions