Yuan-Seng Wu

4 Questions 10 Answers 0 Followers

Questions related from Yuan-Seng Wu

How to count CAG repeats length in a DNA sequence?

Hi, Referring to the above matter, I would like to know the contribution of the CAG repeats length to the risk of developing prostate cancer. In this regards, I am interested in counting...

02 September 2016 6,720 2 View

Can a silenced/knockdowned gene be reactivated/restored after a treatment?

Hi, I would like to ask whether a silenced/knockdown gene mediated with siRNA can be reactivated/restored? I have knockdowned Nrf2 transcription factor (a master regulator of oxidative stress)...

28 March 2016 2,109 11 View

How can I optimize transwell cell invasion assay?

I have tried to do transwell cell invasion assay and encountered a problem, in which after 24 hours of incubation in DMEM complete media (10% FBS) only a few cells (PANC-1 pancreatic cancer cell...

15 August 2015 3,658 10 View

Scratch assay; How can we determine the wound closure is due to cell migration and not cell proliferation?

Hi, I have a question, how do we determine the wound closure in scratch assay is due to cell migration and not cell proliferation? How can we rule out this? If I am not mistaken incubation period...

03 August 2015 9,175 14 View